| Literature DB >> 5158906 |
Abstract
The polysaccharide levan was synthesized in a solidified agar medium containing sucrose as a source of fructose. The biosynthesis was achieved by the enzyme levansucrase (2,6-fructan-d-glucose 6-fructosyltransferase, EC 2.4.1.10), a small quantity of which was placed in circular wells cut in the agar gel. The enzyme slowly diffused through the agar-sucrose medium and the synthesis of levan was observed as circular white areas, the size of which was dependent on the time of incubation and the concentration of enzyme used.Entities:
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Year: 1971 PMID： 5158906 PMCID： PMC1178041 DOI： 10.1042/bj1250209
Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857