Literature DB >> 5154892

Separation of two hemolysins from Aeromonas hydrophila by isoelectric focusing.

B Wretlind, R Möllby, T Wadström.   

Abstract

Two hemolytic proteins (hemolysin I and II) with isoelectric points of 5.5 and 4.3 were purified by isoelectric focusing from the culture supernatant fluid of Aeromonas hydrophila. The purified hemolysins were unstable after dialysis and upon heating at 37 C and were inactivated by proteolytic enzymes. The two hemolysins were lethal for mice in a dose of 10 mug (hemolysin I) and 3 mug (hemolysin II) and showed similar hemolytic spectra on red cells of different species. Both were also cytotoxic for HeLa cells and human fibroblasts and gave dermonecrosis in rabbits.

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Year:  1971        PMID： 5154892      PMCID： PMC416338          DOI： 10.1128/iai.4.4.503-505.1971

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  11 in total

1.  Effect of the hemolysin of Pseudomonas aeruginosa on phosphatides and on phospholipase c activity.

Authors:  S Kurioka; P V Liu
Journal:  J Bacteriol       Date:  1967-02       Impact factor: 3.490

2.  The roles of various fractions of Pseudomonas aeruginosa in its pathogenesis. 3. Identity of the lethal toxins produced in vitro and in vivo.

Authors:  P V Liu
Journal:  J Infect Dis       Date:  1966-10       Impact factor: 5.226

3.  Biological and immunochemical properties of culture filtrates of virulent and avirulent strains of Pseudomonas aeruginosa.

Authors:  S A Carney; R J Jones
Journal:  Br J Exp Pathol       Date:  1968-10

4.  The genus aeromonas in human bacteriology; report of 30 cases and review of the literature.

Authors:  A Von Graevenitz; A H Mensch
Journal:  N Engl J Med       Date:  1968-02-01       Impact factor: 91.245

5.  Formation of bacteriolytic enzymes in batch and continuous culture of Staphylococcus aureus.

Authors:  S Arvidson; T Holme; T Wadström
Journal:  J Bacteriol       Date:  1970-10       Impact factor: 3.490

6.  Studies on extracellular proteins from Staphylococcus aureus. IV. Separation of alpha-toxin by isoelectric focusing.

Authors:  T Wadström
Journal:  Biochim Biophys Acta       Date:  1968-10-21

7.  Studies on extracellular PROTEINS FROM Staphylococcus aureus. I. Separation and characterization of enzymes and toxins by isoelectric focusing.

Authors:  O Vesterberg; T Wadström; K Vesterberg; H Svensson; B Malmgren
Journal:  Biochim Biophys Acta       Date:  1967-04-11

8.  Bacteriolytic enzymes from Staphylococcus aureus. Properties of the endo-beta-N-acetylglucosaminidase.

Authors:  T Wadström
Journal:  Biochem J       Date:  1970-12       Impact factor: 3.857

9.  PARTIAL PURIFICATION OF THE EXTRACELLULAR HEMOLYSIN OF PSEUDOMONAS AERUGINOSA.

Authors:  R S BERK
Journal:  J Bacteriol       Date:  1964-09       Impact factor: 3.490

10.  In Vivo Studies with the Partially Purified Protease (Elastase) from Pseudomonas aeruginosa.

Authors:  G Meinke; J Barum; B Rosenberg; R Berk
Journal:  Infect Immun       Date:  1970-11       Impact factor: 3.441
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  17 in total

1.  Interactions between aerolysin, erythrocytes, and erythrocyte membranes.

Authors:  A W Bernheimer; L S Avigad; G Avigad
Journal:  Infect Immun       Date:  1975-06       Impact factor: 3.441

2.  Aeromonas hydrophila in acute diarrheal disease: detection of enterotoxin and biotyping of strains.

Authors:  A Ljungh; M Popoff; T Wadstrom
Journal:  J Clin Microbiol       Date:  1977-08       Impact factor: 5.948

3.  Intracellular accumulation of extracellular proteins by pleiotropic export mutants of Aeromonas hydrophila.

Authors:  S P Howard; J T Buckley
Journal:  J Bacteriol       Date:  1983-04       Impact factor: 3.490

4.  Characterization of three Aeromonas and nine pseudomonas species by extracellular enzymes and haemolysins.

Authors:  C E Nord; L Sjöberg; T Wadström; B Wretlind
Journal:  Med Microbiol Immunol       Date:  1975       Impact factor: 3.402

Review 5.  Clinical involvement of Aeromonas hydrophila.

Authors:  T J Trust; D C Chipman
Journal:  Can Med Assoc J       Date:  1979-04-21       Impact factor: 8.262

6.  Purification and characterization of an Aeromonas hydrophila hemolysin.

Authors:  T Asao; S Kozaki; K Kato; Y Kinoshita; K Otsu; T Uemura; G Sakaguchi
Journal:  J Clin Microbiol       Date:  1986-08       Impact factor: 5.948

7.  Pathogenicity ofAeromonas hydrophila in red leg disease in frogs.

Authors:  M M Rigney; J W Zilinsky; M A Rouf
Journal:  Curr Microbiol       Date:  1978       Impact factor: 2.188

8.  Cultural conditions for Aeromonas hydrophila affect the production of haemolysins with differing host specificities.

Authors:  B C Nzeako; T S Hastings; A E Ellis
Journal:  Antonie Van Leeuwenhoek       Date:  1991-08       Impact factor: 2.271

9.  Enteropathogenicity of Aeromonas hydrophila and Plesiomonas shigelloides: prevalence among individuals with and without diarrhea in Thailand.

Authors:  C Pitarangsi; P Echeverria; R Whitmire; C Tirapat; S Formal; G J Dammin; M Tingtalapong
Journal:  Infect Immun       Date:  1982-02       Impact factor: 3.441

10.  Membrane-damaging and cytotoxic effects on human fibroblasts of alpha- and beta-hemolysins from Aeromonas hydrophila.

Authors:  M Thelestam; A Ljungh
Journal:  Infect Immun       Date:  1981-12       Impact factor: 3.441
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