Literature DB >> 5132099

Microbial esterase detection with ultraviolet fluorescence.

S K Pancholy, J Q Lynd.   

Abstract

A method is presented to identify esterase-synthesizing microbial colonies within mixed culture plates by utilizing induced esterase hydrolysis of nonfluorescent butyryl ester of 7-hydroxy-4-methylcoumarin to the highly fluorescent 7-hydroxy-4 methyl umbelliferone. Microscopy procedures for making esterase loci of fungal mycelia visible with this reaction are described.

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Year:  1971        PMID： 5132099      PMCID： PMC376453          DOI： 10.1128/am.22.5.939-941.1971

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  4 in total

1.  FLUORESCENCE POLARIZATION: MEASUREMENT WITH ULTRAVIOLET-POLARIZING FILTERS IN A SPECTROPHOTOFLUOROMETER.

Authors:  R F CHEN; R L BOWMAN
Journal:  Science       Date:  1965-02-12       Impact factor: 47.728

2.  Hepatic cholesterol-lipid anomalies induced by aflatoxin.

Authors:  J Q Lynd; F T Lynd
Journal:  Pathol Vet       Date:  1970

3.  Soil microfloral interactions with aflatoxin synthesis and degradation.

Authors:  C M Chang; J Q Lynd
Journal:  Mycologia       Date:  1970 Sep-Oct       Impact factor: 2.696

4.  Fluorometric assay for the hydrolytic activity of lipase using fatty acyl esters of 4-methylumbelliferone.

Authors:  T J Jacks; H W Kircher
Journal:  Anal Biochem       Date:  1967-11       Impact factor: 3.365
  4 in total
  3 in total

Review 1.  Fluorogenic and chromogenic substrates used in bacterial diagnostics.

Authors:  M Manafi; W Kneifel; S Bascomb
Journal:  Microbiol Rev       Date:  1991-09

2.  Microbial Decomposition in Aquatic Environments: Combined Process of Extracellular Enzyme Activity and Substrate Uptake.

Authors:  Hans-Georg Hoppe; Sang-Jin Kim; Klaus Gocke
Journal:  Appl Environ Microbiol       Date:  1988-03       Impact factor: 4.792

3.  A novel chromogenic ester agar medium for detection of Salmonellae.

Authors:  V M Cooke; R J Miles; R G Price; A C Richardson
Journal:  Appl Environ Microbiol       Date:  1999-02       Impact factor: 4.792
  3 in total

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