Literature DB >> 5132092

Improved method for virus structural polypeptide analysis on dissociating acylamide gel.

P A Boulanger, R Warocquier.   

Abstract

A method is described in which polypeptides can be separated, with a high band resolution, by electrophoresis through "pore gradient" acrylamide gel (15 cm in length) in a sodium dodecyl sulfate-urea dissociating system. The applicability of this technique to the analysis of virus structural components was examined with the adenovirus type 2 model system.

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Year:  1971        PMID： 5132092      PMCID： PMC376413          DOI： 10.1128/am.22.5.760-762.1971

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  12 in total

1.  A solubilizable acrylamide gel for electrophoresis.

Authors:  H S. Anker
Journal:  FEBS Lett       Date:  1970-04-16       Impact factor: 4.124

2.  Capsid proteins of Simian virus 40.

Authors:  M Girard; L Marty; F Suarez
Journal:  Biochem Biophys Res Commun       Date:  1970-07-13       Impact factor: 3.575

3.  Isolation of an arginine-rich protein from particles of adenovirus type 2.

Authors:  W G Laver
Journal:  Virology       Date:  1970-07       Impact factor: 3.616

4.  Evidence for differences in size and composition of the poliovirus-specific polypeptides in infected HeLa cells.

Authors:  J V Maizel; D F Summers
Journal:  Virology       Date:  1968-09       Impact factor: 3.616

5.  Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.

Authors:  A L Shapiro; E Viñuela; J V Maizel
Journal:  Biochem Biophys Res Commun       Date:  1967-09-07       Impact factor: 3.575

6.  The polypeptides of adenovirus. II. Soluble proteins, cores, top components and the structure of the virion.

Authors:  J V Maizel; D O White; M D Scharff
Journal:  Virology       Date:  1968-09       Impact factor: 3.616

7.  Structural proteins of Semliki Forest virus and its nucleocapsid.

Authors:  N H Acheson; I Tamm
Journal:  Virology       Date:  1970-06       Impact factor: 3.616

8.  The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.

Authors:  U E Loening
Journal:  Biochem J       Date:  1967-01       Impact factor: 3.857

9.  Pore gradient electrophoresis.

Authors:  D Rodbard; G Kapadia; A Chrambach
Journal:  Anal Biochem       Date:  1971-03       Impact factor: 3.365

10.  Factors affecting resolution, band width, number of theoretical plates, and apparent diffusion coefficients in polyacrylamide gel electrophoresis.

Authors:  J Lunney; A Chrambach; D Rodbard
Journal:  Anal Biochem       Date:  1971-03       Impact factor: 3.365
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  1 in total

1.  Adenovirus protein metabolism at non permissive temperature. II. Transfer of viral polypeptides to nucleus and evidence for a thermostable infected cell specific polypeptide.

Authors:  R Warocquier; P A Boulanger
Journal:  Arch Gesamte Virusforsch       Date:  1973
  1 in total

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