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Literature DB >> 513200

Purification of the JS-3 isolate of Herpesvirus ovis (Bovid herpesvirus 4) and some properties of its DNA.

Abstract

A procedure incorporating the use of heparin was developed to purify Herpesvirus ovis. The viral DNA has a buoyant density of 1.706 +/- 0.001 g/cm3, and the sedimentation constant was estimated to be 47.5 +/- 1.5S; from the latter, the molecular weight was calculated as 67.3 +/- 5.4 X 10(6). Estimates of the guanine-plus-cytosine content made from the buoyant density and melting point (72 degrees C) gave levels of 47 and 46%, respectively.

Entities: Chemical Species

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Year: 1979 PMID： 513200 PMCID： PMC525916

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

28 in total

1. Sedimentation rate as a measure of molecular weight of DNA.

Authors: E BURGI; A D HERSHEY
Journal: Biophys J Date: 1963-07 Impact factor: 4.033

2. THE EFFECT OF HEPARIN ON HERPES SIMPLEX VIRUS.

Authors: A VAHERI; K CANTELL
Journal: Virology Date: 1963-12 Impact factor: 3.616

3. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.

Authors: C L SCHILDKRAUT; J MARMUR; P DOTY
Journal: J Mol Biol Date: 1962-06 Impact factor: 5.469

4. A new method for the isolation of herpes simplex virus type 2 DNA.

Authors: J M Walboomers; J T Schegget
Journal: Virology Date: 1976-10-01 Impact factor: 3.616

5. Isolation of herpes simplex virus DNA from the "hirt supernatant".

Authors: M M Pater; R W Hyman; F Rapp
Journal: Virology Date: 1976-12 Impact factor: 3.616

6. The serological relationship of herpesvirus ovis to other herpesviruses and its possible involvement in the aetiology of jaagsiekte.

Authors: D W Verwoerd; E Meyer-Scharrer; J Broekman; E De Villiers
Journal: Onderstepoort J Vet Res Date: 1979-03 Impact factor: 1.792

Review 1. The family Herpesviridae: an update. The Herpesvirus Study Group of the International Committee on Taxonomy of Viruses.

Authors: B Roizmann; R C Desrosiers; B Fleckenstein; C Lopez; A C Minson; M J Studdert
Journal: Arch Virol Date: 1992 Impact factor: 2.574

Review 2. Animal cytomegaloviruses.

Authors: J Staczek
Journal: Microbiol Rev Date: 1990-09

2 in total

Purification of the JS-3 isolate of Herpesvirus ovis (Bovid herpesvirus 4) and some properties of its DNA.

1. Sedimentation rate as a measure of molecular weight of DNA.

2. THE EFFECT OF HEPARIN ON HERPES SIMPLEX VIRUS.

3. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.

4. A new method for the isolation of herpes simplex virus type 2 DNA.

5. Isolation of herpes simplex virus DNA from the "hirt supernatant".

6. The serological relationship of herpesvirus ovis to other herpesviruses and its possible involvement in the aetiology of jaagsiekte.

7. Classification and nomenclature of viruses. Second report of the International Committee on Taxonomy of Viruses.

8. In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain.

Review 9. Unity and diversity in the herpesviruses.

10. Human cytomegalovirus: purification of enveloped virions and dense bodies.

Review 1. The family Herpesviridae: an update. The Herpesvirus Study Group of the International Committee on Taxonomy of Viruses.

Review 2. Animal cytomegaloviruses.