Literature DB >> 5126468

Catalytic activity of -chymotrypsin in which histidine-57 has been methylated.

R Henderson.   

Abstract

The properties of a derivative of alpha-chymotrypsin in which histidine-57 has been methylated have been examined. Although the modified enzyme binds substrate with the same affinity as does native alpha-chymotrypsin, acylation and deacylation occur at much decreased rates. As for native alpha-chymotrypsin, a basic group of pK(a) approx. 7 is involved in both acylation and deacylation. The significance of these results is considered in relation to the normal function of histidine-57.

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Year:  1971        PMID： 5126468      PMCID： PMC1177107          DOI： 10.1042/bj1240013

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  14 in total

1.  Direct evidence for the presence of histidine in the active center of chymotrypsin.

Authors:  G SCHOELLMANN; E SHAW
Journal:  Biochemistry       Date:  1963 Mar-Apr       Impact factor: 3.162

2.  Three-dimensional structure of tosyl-alpha-chymotrypsin.

Authors:  B W Matthews; P B Sigler; R Henderson; D M Blow
Journal:  Nature       Date:  1967-05-13       Impact factor: 49.962

3.  Optical properties and the chemical nature of acyl-chymotrypsin linkages.

Authors:  E Charney; S A Bernhard
Journal:  J Am Chem Soc       Date:  1967-05-24       Impact factor: 15.419

4.  Structure of crystalline alpha-chymotrypsin. 3. Crystallographic studies of substrates and inhibitors bound to the active site of alpha-chymotrypsin.

Authors:  T A Steitz; R Henderson; D M Blow
Journal:  J Mol Biol       Date:  1969-12-14       Impact factor: 5.469

5.  Methylation of histidine-57 in alpha-chymotrypsin by methyl p-nitrobenzenesulfonate. A new approach to enzyme modification.

Authors:  Y Nakagawa; M L Bender
Journal:  Biochemistry       Date:  1970-01-20       Impact factor: 3.162

6.  Modification of alpha-chymotrypsin by methyl p-nitrobenzenesulfonate.

Authors:  Y Nakagawa; M L Bender
Journal:  J Am Chem Soc       Date:  1969-03-12       Impact factor: 15.419

7.  Structure of crystalline -chymotrypsin. II. A preliminary report including a hypothesis for the activation mechanism.

Authors:  P B Sigler; D M Blow; B W Matthews; R Henderson
Journal:  J Mol Biol       Date:  1968-07-14       Impact factor: 5.469

8.  Role of a buried acid group in the mechanism of action of chymotrypsin.

Authors:  D M Blow; J J Birktoft; B S Hartley
Journal:  Nature       Date:  1969-01-25       Impact factor: 49.962

9.  Implication of an ionizing group in the control of conformation and activity of chymotrypsin.

Authors:  H L Oppenheimer; B Labouesse; G P Hess
Journal:  J Biol Chem       Date:  1966-06-10       Impact factor: 5.157

10.  On the interaction of the active side of alpha-chymotrypsin with chromophores: proflavin binding and enzyme conformation during catalysis.

Authors:  S A Bernhard; B F Lee; Z H Tashjian
Journal:  J Mol Biol       Date:  1966-07       Impact factor: 5.469
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6.  Molecular orbital studies of enzyme activity: catalytic mechanism of serine proteinases.

Authors:  S Scheiner; W N Lipscomb
Journal:  Proc Natl Acad Sci U S A       Date:  1976-02       Impact factor: 11.205

7.  Breaching the conformational integrity of the catalytic triad of the serine protease plasmin: localized disruption of a side chain of His-603 strongly inhibits the amidolytic activity of human plasmin.

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9.  Induction of bladder cancer by N-nitroso-dibutylamine and N-nitrososo-butanol-(4)-butylamine.

Authors:  G F Kolar
Journal:  Br J Cancer       Date:  1972-12       Impact factor: 7.640
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