Literature DB >> 5113489

The purification and properties of the L-serine O-sulphate degrading system of pig liver.

N Tudball, P Thomas, R Bailey-Wood.   

Abstract

1. The enzyme system from pig liver responsible for the alphabeta-elimination of l-serine O-sulphate was purified 1000-fold. 2. Isoelectric focusing produced two enzymically active fractions with isoelectric points at pH5.6 and 5.9 respectively. 3. Osmometry and gel filtration showed both enzymes to possess molecular weights of approx. 54000. 4. The separate activities exhibited similar amino acid compositions.

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Year:  1971        PMID： 5113489      PMCID： PMC1176662          DOI： 10.1042/bj1210747

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  11 in total

1.  A kinetic and equilibrium analysis of the glutamic oxaloacetate transaminase mechanism.

Authors:  S F VELICK; J VAVRA
Journal:  J Biol Chem       Date:  1962-07       Impact factor: 5.157

2.  O-sulphate esters of L-serine, L-threonine and L-hydroxyproline.

Authors:  K S DODGSON; A G LLOYD; N TUDBALL
Journal:  Biochem J       Date:  1961-04       Impact factor: 3.857

3.  Enzymic desulphation of l-serine O[S]-sulphate: the intracellular localization of the enzyme.

Authors:  K S Dodgson; N Tudball
Journal:  Biochem J       Date:  1961-10       Impact factor: 3.857

4.  The metabolism of potassium l-serine O[S]-sulphate in the rat.

Authors:  N Tudball
Journal:  Biochem J       Date:  1962-12       Impact factor: 3.857

5.  The spectrophotometric determination of tyrosine and tryptophan in proteins.

Authors:  T W Goodwin; R A Morton
Journal:  Biochem J       Date:  1946       Impact factor: 3.857

6.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

7.  The reaction of L-serine O-sulfate with aspartate aminotransferase.

Authors:  R A John; P Fasella
Journal:  Biochemistry       Date:  1969-11       Impact factor: 3.162

8.  Studies on the enzymic degradation of L-serine O-sulphate by a rat liver preparation.

Authors:  J H Thomas; N Tudball
Journal:  Biochem J       Date:  1967-11       Impact factor: 3.857

9.  Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. IV. Further studies on the resolving power in connection with separation of myoglobins.

Authors:  O Vesterberg; H Svensson
Journal:  Acta Chem Scand       Date:  1966

10.  A rat liver system that catalyses a pyridoxal phosphate-independent alpha beta-eliminto.

Authors:  J H Thmas; J A Fowler
Journal:  Biochem J       Date:  1969-09       Impact factor: 3.857
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  3 in total

1.  Studies on the L-serine O-sulphate-degrading activity of pig liver.

Authors:  N Tudball; P Thomas
Journal:  Biochem J       Date:  1972-07       Impact factor: 3.857

2.  The enzymic degradation of L-serine O-sulphate. Mechanism of the reaction.

Authors:  N Tudball; P Thomas
Journal:  Biochem J       Date:  1972-06       Impact factor: 3.857

3.  The enzymic degradation of L-serine O-sulphate by a specific system from pig liver. Studies on the mechanism of the reaction.

Authors:  N Tudball; P Thomas
Journal:  Biochem J       Date:  1972-01       Impact factor: 3.857
  3 in total

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