Literature DB >> 508755

The maxi-circle of Trypanosoma brucei kinetoplast DNA.

P Borst, F Fase-Fowler.   

Abstract

We have constructed a fragment map of the maxi-circle component from kinetoplast DNA networks of Trypanosoma brucei brucei (East African Trypanosomiasis Research Organization strain No. 427), using restriction endonucleases PstI, HapII, EcoRI, BglI, HindIII, HhaI, SstI, XbaI, HaeIII and Sau-96I. Although the 20 kilo-base pair map contains 30 fragments, there is a 6.5 kilo-base pair 'silent' segment which lacks recognition sites for any of these enzymes. In CsCl intact kinetoplast DNA has a buoyant density of 1.690 g/cm3, whereas a kinetoplast DNA fraction enriched in maxi-circle sequences contains an additional component at 1.682 g/cm3. The possibility that the 'silent' segment is very rich in A + T is discussed.

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Year:  1979        PMID: 508755     DOI: 10.1016/0005-2787(79)90078-9

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN: 0006-3002


  13 in total

1.  RNA editing in the free-living bodonid Bodo saltans.

Authors:  D Blom; A de Haan; M van den Berg; P Sloof; M Jirku; J Lukes; R Benne
Journal:  Nucleic Acids Res       Date:  1998-03-01       Impact factor: 16.971

2.  Characterization of the DNA duplication-transposition that controls the expression of two genes for variant surface glycoproteins in Trypanosoma brucei.

Authors:  L H Van der Ploeg; A Bernards; F A Rijsewijk; P Borst
Journal:  Nucleic Acids Res       Date:  1982-01-22       Impact factor: 16.971

3.  An analysis of cosmid clones of nuclear DNA from Trypanosoma brucei shows that the genes for variant surface glycoproteins are clustered in the genome.

Authors:  L H Van der Ploeg; D Valerio; T De Lange; A Bernards; P Borst; F G Grosveld
Journal:  Nucleic Acids Res       Date:  1982-10-11       Impact factor: 16.971

4.  Characterization of the expression-linked gene copies of variant surface glycoprotein 118 in two independently isolated clones of Trypanosoma brucei.

Authors:  P A Michels; A Bernards; L H Van der Ploeg; P Borst
Journal:  Nucleic Acids Res       Date:  1982-04-10       Impact factor: 16.971

5.  Comparison of maxicircle DNAs of Leishmania tarentolae and Trypanosoma brucei.

Authors:  M L Muhich; L Simpson; A M Simpson
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

Review 6.  Structure and evolution of organelle genomes.

Authors:  D C Wallace
Journal:  Microbiol Rev       Date:  1982-06

7.  Cloning and characterization of two guide RNA-binding proteins from mitochondria of Crithidia fasciculata: gBP27, a novel protein, and gBP29, the orthologue of Trypanosoma brucei gBP21.

Authors:  D Blom; C K Breek; D Speijer; A O Muijsers; R Benne
Journal:  Nucleic Acids Res       Date:  2001-07-15       Impact factor: 16.971

8.  Mitochondrial minicircles in the free-living bodonid Bodo saltans contain two gRNA gene cassettes and are not found in large networks.

Authors:  D Blom; A de Haan; J van den Burg; M van den Berg; P Sloof; M Jirku; J Lukes; R Benne
Journal:  RNA       Date:  2000-01       Impact factor: 4.942

9.  RNA editing in transcripts of the mitochondrial genes of the insect trypanosome Crithidia fasciculata.

Authors:  H Van der Spek; D Speijer; G J Arts; J Van den Burg; H Van Steeg; P Sloof; R Benne
Journal:  EMBO J       Date:  1990-01       Impact factor: 11.598

10.  Transcripts from the frameshifted MURF3 gene from Crithidia fasciculata are edited by U insertion at multiple sites.

Authors:  H van der Spek; J van den Burg; A Croiset; M van den Broek; P Sloof; R Benne
Journal:  EMBO J       Date:  1988-08       Impact factor: 11.598

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