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Literature DB >> 507796

Use of covalently closed circular deoxyribonucleic acid for prescreening for antitumor compounds.

S Mong, J E Strong, J A Bush, S T Crooke.

Abstract

The interactions of PM-2 deoxyribonucleic acid (DNA) with 20 known antineoplastic agents were studied using agarose gel electrophoresis and fluorescence assay systems. Actinomycin D and adriamycin (and all other anthracyclines studied) induced superhelical conformational changes in PM-2 DNA. Bleomycin A2, tallysomycin, neocarzinostatin, macromomycin, and hedamycin degraded PM-2 DNA under various conditions. The potential utility of PM-2 DNA as an antitumor antibiotic prescreening tool was further studied by examining the effects of 200 broths on PM-2 DNA employing both fluorescence and agarose gel electrophorectic assays. The assays were shown to be sensitive and rapid and to provide information not provided by other prescreens with which the PM-2 DNA assays were compared.

Entities: Chemical

Mesh：

Substances：

Year: 1979 PMID： 507796 PMCID： PMC352867 DOI： 10.1128/AAC.16.3.398

Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN： 0066-4804 Impact factor: 5.191

16 in total

1. SYSTEM FOR DETECTING INDUCERS OF LYSOGENIC ESCHERICHIA COLI W1709 (LAMBDA) AND ITS APPLICABILITY AS A SCREEN FOR ANTINEOPLASTIC ANTIBIOTICS.

Authors: K E PRICE; R E BUCK; J LEIN
Journal: Appl Microbiol Date: 1964-09

2. Microbiological assays and bioautography of maytansine and its homologues.

Authors: L J Hanka; M S Barnett
Journal: Antimicrob Agents Chemother Date: 1974-11 Impact factor: 5.191

3. Strand scission of DNA by bound adriamycin and daunorubicin in the presence of reducing agents.

Authors: J W Lown; S K Sim; K C Majumdar; R Y Chang
Journal: Biochem Biophys Res Commun Date: 1977-06-06 Impact factor: 3.575

4. Electrophoresis of DNA in agarose gels. Optimizing separations of conformational isomers of double- and single-stranded DNAs.

Authors: P H Johnson; L I Grossman
Journal: Biochemistry Date: 1977-09-20 Impact factor: 3.162

Use of covalently closed circular deoxyribonucleic acid for prescreening for antitumor compounds.

1. SYSTEM FOR DETECTING INDUCERS OF LYSOGENIC ESCHERICHIA COLI W1709 (LAMBDA) AND ITS APPLICABILITY AS A SCREEN FOR ANTINEOPLASTIC ANTIBIOTICS.

2. Microbiological assays and bioautography of maytansine and its homologues.

3. Strand scission of DNA by bound adriamycin and daunorubicin in the presence of reducing agents.

4. Electrophoresis of DNA in agarose gels. Optimizing separations of conformational isomers of double- and single-stranded DNAs.

5. Bristol Laboratories Division, Bristol-Myers Company, Syracuse, N.Y.

6. DNA breakage by tallysomycin.

7. DNA double-strand breaks and alkali-labile bonds produced by bleomycin.

8. Two actions of bleomycin on superhelical DNA.

9. The mechanism of the bleomycin-induced cleavage of DNA.

10. Bleomycin-specific fragmentation of double-stranded DNA.

1. Mechanism of action of dnacin B1, a new benzoquinoid antibiotic with antitumor properties.

Review 2. Advances in platinum cancer chemotherapy. Advances in the design of cisplatin analogues.