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Literature DB >> 5061986

Genome of Sindbis virus.

Abstract

(32)P-labeled ribonucleic acid (RNA) from purified Sindbis virus was examined for the presence of hidden breaks. Viral RNA was treated with acid at pH 2.9 or with formaldehyde and was analyzed on sucrose gradients or by polyacrylamide gel electrophoresis. The sedimentation pattern and mobility on polyacrylamide gels of the 42S RNA was unaffected by heating and quick cooling and was not altered by denaturing agents such as dimethyl sulfoxide and urea. No evidence that Sindbis RNA is a polyaggregate of fragments was obtained. It is concluded that the genome consists of a continuous length of single-stranded polynucleotide.

Entities: Chemical Species

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Year: 1972 PMID： 5061986 PMCID： PMC356268

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

16 in total

1. The effect of reaction with formaldehyde on the sedimentation rates of ribonucleic acids.

Authors: M L Fenwick
Journal: Biochem J Date: 1968-05 Impact factor: 3.857

2. Visualisation in the electron microscope and size of RNA from animal cells.

Authors: N Granboulan; K Scherrer
Journal: Eur J Biochem Date: 1969-05-01

3. Differences between the structures of poliovirus and Sindbis virus infectious ribonucleic acids.

Authors: S Sprecher-Goldberger
Journal: Arch Gesamte Virusforsch Date: 1967

4. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.

Authors: A C Peacock; C W Dingman
Journal: Biochemistry Date: 1968-02 Impact factor: 3.162

5. The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation.

Authors: U E Loening
Journal: Biochem J Date: 1969-06 Impact factor: 3.857

6. Togavirus RNA: reversible effect of urea on genomes and absence of subgenomic viral RNA in Kunjin virus-infected cells.

Authors: R W Boulton; E G Westaway
Journal: Arch Virol Date: 1977 Impact factor: 2.574

Genome of Sindbis virus.

1. The effect of reaction with formaldehyde on the sedimentation rates of ribonucleic acids.

2. Visualisation in the electron microscope and size of RNA from animal cells.

3. Differences between the structures of poliovirus and Sindbis virus infectious ribonucleic acids.

4. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.

5. The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation.

6. A kinetic analysis of the synthesis in BHK 21 cells of RNAs specific for Semliki Forest virus.

7. Denaturation of Sindbis virus RNA with dimethyl sulphoxide.

8. Characterization of a cytoplasmic fraction from Sindbis virus-infected cultures.

9. Properties of 42S and 26S Sindbis viral ribonucleic acid species.

10. Molecular weight of Sindbis virus ribonucleic acid as measured by polyacrylamide gel electrophoresis.

1. Genetic exclusion and stable complementation of Sindbis virus.

2. Size and structure of the genome of infectious pancreatic necrosis virus.

3. Molecular weight determination of Sendai and Newcastle disease virus RNA.

4. Virus-directed post-translational cleavage in Sindbus virus-infected cells.

5. Characterization of virus-specific RNA synthesized in bovine cells infected with bovine viral diarrhea virus.

6. Togavirus RNA: reversible effect of urea on genomes and absence of subgenomic viral RNA in Kunjin virus-infected cells.

7. Molecular weight determination of Sendai RNA by dimethyl sulfoxide gradient sedimentation.

8. Gamma ray-induced small plaque mutants of western equine encephalitis virus.

9. Semliki forest virus-specific RNAs synthesized in vitro by enzyme from infected BHK cells.

10. Identification of polysomal RNA in BHK cells infected by sindbis virus.