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Literature DB >> 50588

Hybridization of pigeon globin messenger RNA with complementary DNA synthesized in vitro by reverse transcription: influence of the homopolymeric regions.

K G Gasaryan, V Z Tarantul, Y N Baranov, L Y Frolova, L L Kisselev.

Abstract

The kinetics of hybridization of pigeon globin messenger RNA with complementary cDNA synthesized by means of AMV reverse transcriptase is complex. Addition of poly A or poly U in excess to the reaction mixture normalized the kinetics. It is concluded that association of the complementary homopolymeric regions of mRNA and cDNA accelerates the complex formation between heteropolymeric sequences in a fraction of the molecules.

Entities: Chemical

Mesh：

Substances：

Year: 1975 PMID： 50588 PMCID： PMC343506 DOI： 10.1093/nar/2.7.1203

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

18 in total

1. Localization of the polyadenylate sequences in messenger ribonucleic acid and in the heterogeneous nuclear ribonucleic acid of HeLa cells.

Authors: H Nakazato; D W Kopp; M Edmonds
Journal: J Biol Chem Date: 1973-02-25 Impact factor: 5.157

2. In vitro synthesis of DNA components of human genes for globins.

Authors: A Bank; M Terada; S Metafora; L Dow; P A Marks
Journal: Nat New Biol Date: 1972-02-09

3. Purification of the DNA polymerase of avian myeloblastosis virus.

Authors: D L Kacian; K F Watson; A Burny; S Spiegelman
Journal: Biochim Biophys Acta Date: 1971-09-24

4. In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA.

Authors: I M Verma; G F Temple; H Fan; D Baltimore
Journal: Nat New Biol Date: 1972-02-09

5. [Enzymatic synthesis of DNA, complemental to pigeon globin mRNA, by reverse transcription].

Authors: L L Kiselev; L Iu Frolova; K G Gazarian; V Z Tarantul; V A Engel'gardt
Journal: Dokl Akad Nauk SSSR Date: 1973-12-11

6. Kinetic studies of gene frequency. I. Use of a DNA copy of reticulocyte 9 S RNA to estimate globin gene dosage in mouse tissues.

Authors: P R Harrison; G D Birnie; A Hell; S Humphries; B D Young; J Paul
Journal: J Mol Biol Date: 1974-04-25 Impact factor: 5.469

Hybridization of pigeon globin messenger RNA with complementary DNA synthesized in vitro by reverse transcription: influence of the homopolymeric regions.

1. Localization of the polyadenylate sequences in messenger ribonucleic acid and in the heterogeneous nuclear ribonucleic acid of HeLa cells.

2. In vitro synthesis of DNA components of human genes for globins.

3. Purification of the DNA polymerase of avian myeloblastosis virus.

4. In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA.

5. [Enzymatic synthesis of DNA, complemental to pigeon globin mRNA, by reverse transcription].

6. Kinetic studies of gene frequency. I. Use of a DNA copy of reticulocyte 9 S RNA to estimate globin gene dosage in mouse tissues.

7. Characterization of synthetic rabbit globin DNA.

8. Synthesis of complementary DNA from lens mRNA with RNA-dependent DNA polymerase.

9. Synthesis of DNA complementary to 14S calf lens crystallin messenger RNA by reverse transcriptase.

10. Pattern of RNA synthesis in duck erythrocytes in relationship to the stage of cell differentiation.

1. Transcription of single base oligonucleotides by ribonucleic acid-directed deoxyribonucleic acid polymerase.