Literature DB >> 5040386

A 2 (N2) neuraminidase of the X-7 influenza virus recombinant: determination of molecular size and subunit composition of the active unit.

D J Bucher, E D Kilbourne.   

Abstract

Neuraminidase activity of influenza virus was directly seen on sodium dodecyl sulfate polyacrylamide gels with the aid of the synthetic substrate, methoxyphenol neuraminic acid. Neuraminidase (NA) appeared as a high-molecular-weight fraction with a size in the range of 220,000 to 250,000 daltons. Isolation of this fraction from the X-7 strain of influenza virus, dissociation with sodium dodecyl sulfate, and reduction showed the presence of two polypeptides of 66,000 (NA(1)) and 58,000 (NA(2)) molecular weights in equimolar concentration. We postulate that the minimum active unit for the viral A(2) neuraminidase is a tetramer composed of two NA(1) and two NA(2) subunits.

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Year:  1972        PMID： 5040386      PMCID： PMC356425     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  15 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

Authors:  R G MARTIN; B N AMES
Journal:  J Biol Chem       Date:  1961-05       Impact factor: 5.157

3.  Related studies of a recombinant influenza-virus vaccine. I. Derivation and characterization of virus and vaccine.

Authors:  E D Kilbourne; J L Schulman; G C Schild; G Schloer; J Swanson; D Bucher
Journal:  J Infect Dis       Date:  1971-11       Impact factor: 5.226

4.  Separation of two polypeptide chains from the hemagglutinin subunit of influenza virus.

Authors:  W G Laver
Journal:  Virology       Date:  1971-07       Impact factor: 3.616

5.  Estimation of the molecular weights of the polypeptide chains from the isolated hemagglutinin and neuraminidase subunits of influenza viruses.

Authors:  R G Webster
Journal:  Virology       Date:  1970-03       Impact factor: 3.616

6.  Gel chromatography of proteins in denaturing solvents. Comparison between sodium dodecyl sulfate and guanidine hydrochloride as denaturants.

Authors:  W W Fish; J A Reynolds; C Tanford
Journal:  J Biol Chem       Date:  1970-10-10       Impact factor: 5.157

7.  The structure of influenza virus. I. The polypeptides of the virion.

Authors:  I T Schulze
Journal:  Virology       Date:  1970-12       Impact factor: 3.616

8.  Quantitative determination of neuraminidase-active foci in cell monolayer cultures infected with influenza or newcastle disease virus.

Authors:  P Palese; G Bodo; H Tuppy
Journal:  J Virol       Date:  1970-10       Impact factor: 5.103

9.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

10.  Identification in a recombinant influenza virus of structural proteins derived from both parents.

Authors:  W G Laver; E D Kilbourne
Journal:  Virology       Date:  1966-11       Impact factor: 3.616
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  24 in total

1.  Biological and protective properties of immune sera directed to the influenza virus neuraminidase.

Authors:  Stefan J Halbherr; Thomas H Ludersdorfer; Meret Ricklin; Samira Locher; Marianne Berger Rentsch; Artur Summerfield; Gert Zimmer
Journal:  J Virol       Date:  2014-11-12       Impact factor: 5.103

2.  An Amino Acid in the Stalk Domain of N1 Neuraminidase Is Critical for Enzymatic Activity.

Authors:  Mark Zanin; Susu Duan; Sook-San Wong; Gyanendra Kumar; Pradyumna Baviskar; Emily Collin; Charles Russell; Subrata Barman; Benjamin Hause; Richard Webby
Journal:  J Virol       Date:  2017-01-03       Impact factor: 5.103

3.  Identification of Residues That Affect Oligomerization and/or Enzymatic Activity of Influenza Virus H5N1 Neuraminidase Proteins.

Authors:  Meiling Dai; Hongbo Guo; Jos C F M Dortmans; Jojanneke Dekkers; Johan Nordholm; Robert Daniels; Frank J M van Kuppeveld; Erik de Vries; Cornelis A M de Haan
Journal:  J Virol       Date:  2016-09-29       Impact factor: 5.103

4.  Applications of a synthetic neuraminidase substrate.

Authors:  P Palese; D Bucher; E D Kilbourne
Journal:  Appl Microbiol       Date:  1973-02

5.  In silico analysis of drug-resistant mutant of neuraminidase (N294S) against oseltamivir.

Authors:  V Karthick; V Shanthi; R Rajasekaran; K Ramanathan
Journal:  Protoplasma       Date:  2012-03-06       Impact factor: 3.356

6.  Steps in maturation of influenza A virus neuraminidase.

Authors:  T Saito; G Taylor; R G Webster
Journal:  J Virol       Date:  1995-08       Impact factor: 5.103

7.  Characterization of a temperature-sensitive influenza B virus mutant defective in neuraminidase.

Authors:  S Shibata; F Yamamoto-Goshima; K Maeno; T Hanaichi; Y Fujita; K Nakajima; M Imai; T Komatsu; S Sugiura
Journal:  J Virol       Date:  1993-06       Impact factor: 5.103

8.  Neuraminidase gene from the early Asian strain of human influenza virus, A/RI/5-/57 (H2N2).

Authors:  T C Elleman; A A Azad; C W Ward
Journal:  Nucleic Acids Res       Date:  1982-11-11       Impact factor: 16.971

9.  Mapping of the influenza virus genome. III. Identification of genes coding for nucleoprotein, membrane protein, and nonstructural protein.

Authors:  M B Ritchey; P Palese; J L Schulman
Journal:  J Virol       Date:  1976-10       Impact factor: 5.103

10.  Characterization of influenza virus neuraminidases: peptide changes associated with antigenic divergence between early and late N2 neuraminidases.

Authors:  A P Kendal; M P Kiley
Journal:  J Virol       Date:  1973-12       Impact factor: 5.103
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