Literature DB >> 5031560

Use of gas chromatography for determining catabolic products of arginine by bacteria.

C W Moss, M A Lambert, W B Cherry.   

Abstract

A rapid and sensitive procedure for determining catabolic products of arginine metabolism by bacteria was developed. The method consists of inoculating a solution of L-arginine with a heavy cell suspension of the test organism. After a 2-hr incubation period, dissimilation products (citrulline, ornithine, agmatine, putrescine) are converted to volatile derivatives and analyzed by gas-liquid chromatography. Compared with conventional microbiological tests, the new procedure is rapid and can be used for sensitive quantitative measurements of specific metabolites from arginine.

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Year:  1972        PMID： 5031560      PMCID： PMC380466          DOI： 10.1128/am.23.5.889-893.1972

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  14 in total

1.  Argenine decarboxylase from Escherichia coli. I. Purification and specificity for substrates and coenzyme.

Authors:  S L Blethen; E A Boeker; E E Snell
Journal:  J Biol Chem       Date:  1968-04-25       Impact factor: 5.157

2.  The occurrence of a catabolic and an anabolic ornithine carbamoyltransferase in Pseudomonas.

Authors:  V Stalon; F Ramos; A Piérard; J M Wiame
Journal:  Biochim Biophys Acta       Date:  1967-05-16

3.  The specialization of the two ornithine carbamoyltransferases of Pseudomonas.

Authors:  F Ramos; V Stalon; A Piérard; J M Wiame
Journal:  Biochim Biophys Acta       Date:  1967-05-16

4.  Gas-liquid chromatography of twenty protein amino acids on a single column.

Authors:  C W Moss; M A Lambert; F J Diaz
Journal:  J Chromatogr       Date:  1971-08-05

5.  Rapid methods for determining decarboxylase activity: arginine decarboxylase.

Authors:  M C Goldschmidt; B M Lockhart
Journal:  Appl Microbiol       Date:  1971-09

6.  Simplified rapid procedure for determination of agmatine and other guanidino-containing compounds.

Authors:  M C Goldschmidt; B M Lockhart
Journal:  Anal Chem       Date:  1971-09       Impact factor: 6.986

7.  Characterization of saccharolytic nonfermentative bacteria associated with man.

Authors:  M J Pickett; M M Pedersen
Journal:  Can J Microbiol       Date:  1970-05       Impact factor: 2.419

8.  Multiple pathways of putrescine biosynthesis in Escherichia coli.

Authors:  D R Morris; A B Pardee
Journal:  J Biol Chem       Date:  1966-07-10       Impact factor: 5.157

9.  The aerobic pseudomonads: a taxonomic study.

Authors:  R Y Stanier; N J Palleroni; M Doudoroff
Journal:  J Gen Microbiol       Date:  1966-05

10.  Characterization of Pseudomonas species isolated from clinical specimens.

Authors:  G L Gilardi
Journal:  Appl Microbiol       Date:  1971-03
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  2 in total

1.  Rapid method for simultaneous detection of the arginine dihydrolase system and amino acid decarboxylases in microorganisms.

Authors:  K C Chen; N J Culbertson; J S Knapp; G E Kenny; K K Holmes
Journal:  J Clin Microbiol       Date:  1982-11       Impact factor: 5.948

2.  Methyl mercaptan and dimethyl disulfide production from methionine by Proteus species detected by head-space gas-liquid chromatography.

Authors:  N J Hayward; T H Jeavons; A J Nicholson; A G Thornton
Journal:  J Clin Microbiol       Date:  1977-09       Impact factor: 5.948
  2 in total

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