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Literature DB >> 502764

A simplified procedure for the determination of betaine in liver.

Abstract

A convenient procedure for the determination of hepatic betaine levels is described. The method takes advantage of ethanol precipitation to rid acidified tissue extracts of interfering substances. Betaine is reacted with potassium triiodide to form betaine periodide, which is selectively precipitated via pH adjustment. The precipitate of betaine periodide is dissolved in ethylene dichloride and measured spectrophotometrically. The method is specific, accurate, and simple and showed recoveries of from 97 to 103% at two different levels of added betaine. The applicability of the method was shown when it was demonstrated that diets containing different amounts of choline influenced levels of hepatic betaine.

Entities: Chemical

Mesh：

Substances：
Betaine

Year: 1979 PMID： 502764 DOI： 10.1007/bf02534129

Source DB: PubMed Journal: Lipids ISSN： 0024-4201 Impact factor: 1.880

12 in total

1. Current considerations regarding reference units in the expression of biomedical data.

Authors: A J Barak; D J Tuma; M F Sorrell
Journal: Life Sci Date: 1975-07-15 Impact factor: 5.037

2. Liver choline oxidase activity in man and in several species of animals.

Authors: H SIDRANSKY; E FARBER
Journal: Arch Biochem Biophys Date: 1960-03 Impact factor: 4.013

3. Quantitative separation and identification of quaternary ammonium bases.

Authors: S FRIEDMAN; J E MCFARLANE; P K BHATTACHARYYA; G FRAENKEL
Journal: Arch Biochem Biophys Date: 1955-12 Impact factor: 4.013

4. Studies of the acute and chronic effects of ethanol ingestion on choline oxidation.

Authors: J A Thompson; R C Reitz
Journal: Ann N Y Acad Sci Date: 1976 Impact factor: 5.691

5. Distribution and metabolism of intravenously administered choline[methyl- 3-H] and synthesis in vivo of acetylcholine in various tissues of guinea pigs.

Authors: D R Haubrich; P F Wang; P W Wedeking
Journal: J Pharmacol Exp Ther Date: 1975-04 Impact factor: 4.030

2. Osmoprotective activity for Escherichia coli in mammalian renal inner medulla and urine. Correlation of glycine and proline betaines and sorbitol with response to osmotic loads.

Authors: S T Chambers; C M Kunin
Journal: J Clin Invest Date: 1987-11 Impact factor: 14.808

3. Bleaching effects of massecuite on some properties of crystallized sugar beet and milk chocolate bars produced.

Authors: F Seyed Sadjadi; M Honarvar; A Kalbasi-Ashtari; P Motaghian
Journal: J Food Sci Technol Date: 2018-02-16 Impact factor: 2.701

4. Investigations on ideal mode of cell disruption in extremely halophilic Actinopolyspora halophila (MTCC 263) for efficient release of glycine betaine and trehalose.

Authors: Jayaranjan R Kar; Rekha S Singhal
Journal: Biotechnol Rep (Amst) Date: 2014-12-18

4 in total

A simplified procedure for the determination of betaine in liver.

1. Current considerations regarding reference units in the expression of biomedical data.

2. Liver choline oxidase activity in man and in several species of animals.

3. Quantitative separation and identification of quaternary ammonium bases.

4. Studies of the acute and chronic effects of ethanol ingestion on choline oxidation.

5. Distribution and metabolism of intravenously administered choline[methyl- 3-H] and synthesis in vivo of acetylcholine in various tissues of guinea pigs.

Review 6. Relationship of ethanol to choline metabolism in the liver: a review.

7. Effect of ethanol on uptake of choline by the isolated perfused rat liver.

8. Possible interrelationship of ethanol metabolism and choline oxidation in the liver.

9. Pathways for the incorporation of choline into rat liver phosphatidylcholines in vivo.

10. Choline oxidation and labile methyl groups in normal and choline-deficient rat liver.

1. Effect of choline deficiency on S-adenosylmethionine and methionine concentrations in rat liver.

2. Osmoprotective activity for Escherichia coli in mammalian renal inner medulla and urine. Correlation of glycine and proline betaines and sorbitol with response to osmotic loads.

3. Bleaching effects of massecuite on some properties of crystallized sugar beet and milk chocolate bars produced.

4. Investigations on ideal mode of cell disruption in extremely halophilic Actinopolyspora halophila (MTCC 263) for efficient release of glycine betaine and trehalose.