Literature DB >> 500726

Ribonuclease inhibitor from human placenta: rapid purification and assay.

P Blackburn.   

Abstract

The ribonuclease inhibitor from human placenta may be isolated in 65% yield (2.5 mg per placenta) in 2 days. The performance of the affinity chromatography on Sepharose-RNase A has been expedited through adaption of the spectrophotometric assay of ribonuclease toward 2',3'-cyclic cytidine monophosphate to determination of the inhibitor activity. The result of these improvements in procedure is increased availability of the ribonuclease inhibitor for study of its chemical properties (Blackburn, P., and Jailkhani, B.L. (1979) J. Biol. Chem. 254, 12488-12493) and for its inclusion into in vitro systems in which inhibition of mammalian neutral ribonucleases is desired.

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Year:  1979        PMID： 500726

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  28 in total

Review 1.  Ribonuclease inhibitor: structure and function.

Authors:  Kimberly A Dickson; Marcia C Haigis; Ronald T Raines
Journal:  Prog Nucleic Acid Res Mol Biol       Date:  2005

2.  Heat shock proteins of higher plants.

Authors:  J L Key; C Y Lin; Y M Chen
Journal:  Proc Natl Acad Sci U S A       Date:  1981-06       Impact factor: 11.205

3.  In-vivo phytochrome control of in vitro transcription rates in isolated nuclei from oat seedlings.

Authors:  E Mösinger; E Schäfer
Journal:  Planta       Date:  1984-07       Impact factor: 4.116

4.  Activation of a neuralizing factor in amphibian ectoderm.

Authors:  Manorama John; Jochen Born; Heinz Tiedemann; Hildegard Tiedemann
Journal:  Wilehm Roux Arch Dev Biol       Date:  1984-01

5.  Modular mutagenesis of human placental ribonuclease inhibitor, a protein with leucine-rich repeats.

Authors:  F S Lee; B L Vallee
Journal:  Proc Natl Acad Sci U S A       Date:  1990-03       Impact factor: 11.205

6.  In Vitro Synthesis and Processing of Wheat alpha-Amylase : TRANSLATION OF GIBBERELLIC ACID-INDUCED WHEAT ALEURONE LAYER RNA BY WHEAT GERM AND XENOPUS LAEVIS OOCYTE SYSTEMS.

Authors:  R S Boston; T J Miller; J E Mertz; R R Burgess
Journal:  Plant Physiol       Date:  1982-01       Impact factor: 8.340

7.  Some enzymatic activities associated with purified parapoxvirions.

Authors:  H S Caplen; J A Holowczak
Journal:  J Virol       Date:  1983-05       Impact factor: 5.103

8.  In vitro release of alpha 1-acid glycoprotein RNA sequences shows fidelity with the acute phase response in vivo.

Authors:  G A Clawson; J Button; C H Woo; Y C Liao; E A Smuckler
Journal:  Mol Biol Rep       Date:  1986       Impact factor: 2.316

9.  Ribonuclease A variants with potent cytotoxic activity.

Authors:  P A Leland; L W Schultz; B M Kim; R T Raines
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-01       Impact factor: 11.205

10.  Human placental ribonuclease inhibitor abolishes both angiogenic and ribonucleolytic activities of angiogenin.

Authors:  R Shapiro; B L Vallee
Journal:  Proc Natl Acad Sci U S A       Date:  1987-04       Impact factor: 11.205
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