| Literature DB >> 4985881 |
J D Karkas, R Rudner, E Chargaff.
Abstract
DNA preparations from seven bacterial species and from E. coli phage T4, and also the complementary L and H fractions into which these DNA specimens, after denaturation, were separated by chromatography on methylated albumin-kieselguhr columns, were studied as templates in the RNA polymerase system, and the nucleotide composition of the RNA products was determined. The RNA transcripts of the separated L and H fractions were found to be faithful copies of the respective DNA fractions. This suggests "transcription analysis" as a sensitive and reliable analytical technique for the determination of the base composition of denatured DNA. The L and H fractions of T4 DNA were shown, both by temperature-absorbance profiles and by transcription analysis, to be mutually complementary. The RNA products formed with intact DNA as the template were not exact copies of the latter; their composition indicated that under our experimental conditions the "heavy" DNA strand is transcribed preferentially.Entities:
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Year: 1970 PMID： 4985881 PMCID： PMC283021 DOI： 10.1073/pnas.65.4.1049
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205