Agglutinin response of rabbits to combined Pasteurella tularensis-Brucella abortus vaccination.

A schedule was developed for the simultaneous production of a Pasteurella tularensis-Brucella abortus antiserum in rabbits. Three doses of 10(8) viable P. tularensis LVS organisms were given intravenously at weekly intervals. One day prior to the final dose of P. tularensis, the rabbits received 10(9) viable cells of B. abortus strain 19 intravenously. The use of live vaccines, administered in this sequence, resulted in high agglutinin titers within 3 weeks. The maximal agglutinin titer to either organism was observed 1 week after the final injection.