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Literature DB >> 4984384

Studies of the aromatic circular dichroism of Staphylococcal nuclease.

Abstract

Specific contributions of tyrosyl and of tryptophanyl residues can be distinguished in the near-ultraviolet circular dichroic spectrum of staphylococcal nuclease. Upon binding of the inhibitor deoxythymidine 3',5'-diphosphate in the presence of Ca(++), a significant change in the circular dichroic spectrum results which has been used to characterize the interaction of ligand and enzyme. The data suggest that the asymmetric environment of certain tyrosyl residues is altered by binding of the nucleotide inhibitor.

Entities: Chemical Disease

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Year: 1969 PMID： 4984384 PMCID： PMC223323 DOI： 10.1073/pnas.64.3.923

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

18 in total

1. Biosynthetic incorporation of tryptophan analogues into staphylococcal nuclease: effect of 5-hydroxytryptophan and 7-azatryptophan on structure and stability.

Authors: C Y Wong; M R Eftink
Journal: Protein Sci Date: 1997-03 Impact factor: 6.725

2. Staphylococcal nuclease reviewed: a prototypic study in contemporary enzymology. II. Solution studies of the nucleotide binding site and the effects of nucleotide binding.

Authors: P W Tucker; E E Hazen; F A Cotton
Journal: Mol Cell Biochem Date: 1979-01-15 Impact factor: 3.396

2 in total

Studies of the aromatic circular dichroism of Staphylococcal nuclease.

1. Catalytic properties and specificity of the extracellular nuclease of Staphylococcus aureus.

2. The interaction of nucleotides with the active site of staphylococcal nuclease. Spectrophotometric studies.

3. Optil rotatory dispersion and circular dichroism of human carbonic anhydrases B and C.

4. Steps in the formation of active derivatives of staphylococcal nuclease during trypsin digestion.

5. The tyrosyl residues at the active site of staphylococcal nuclease. Modifications by tetranitromethane.

Review 6. Circular dichroism of biological macromolecules.

7. Fluorescence studies of the interaction of nucleotides with the active site of the nuclease of Staphylococcus aureus.

8. Side-chain Cotton effects of ribonuclease.

9. An analysis of the tyrosine circular dichroism bands in ribonuclease.

10. High-resolution nuclear magnetic resonance spectra of selectively deuterated staphylococcal nuclease.

1. Biosynthetic incorporation of tryptophan analogues into staphylococcal nuclease: effect of 5-hydroxytryptophan and 7-azatryptophan on structure and stability.

2. Staphylococcal nuclease reviewed: a prototypic study in contemporary enzymology. II. Solution studies of the nucleotide binding site and the effects of nucleotide binding.