Literature DB >> 4975152

Electrophoretic characteristics of staphylococcal hyaluronate lyase.

C Abramson, H Friedman.   

Abstract

Partially purified staphylococcal hyaluronidase was studied with respect to its electrophoretic properties by the use of starch slurry or semisolid Noble agar as a matrix. The polarity of enzyme activity was found to be cathodal on starch and anodal on agar gel. The electrophoretic migration of the partially purified enzyme on starch, as a function of pH, suggested that the enzyme has an isoelectric point of pH 9.5 to 10.

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Year:  1969        PMID： 4975152      PMCID： PMC249750          DOI： 10.1128/jb.97.2.715-718.1969

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  9 in total

1.  FACTORS INFLUENCING THE ELECTROPHORETIC MIGRATION OF LACTIC DEHYDROGENASE ISOZYMES.

Authors:  N RESSLER; J SCHULZ; R R JOSEPH
Journal:  J Lab Clin Med       Date:  1963-10

2.  Adsorption of proteins on 'Sephadex'.

Authors:  A N GLAZER; D WELLNER
Journal:  Nature       Date:  1962-06-02       Impact factor: 49.962

3.  Agarose as an anticonvection agent in zone electrophoresis.

Authors:  S HJERTEN
Journal:  Biochim Biophys Acta       Date:  1961-11-11

4.  Studies on diffusing factors: 2. Methods of assay of hyaluronidase and their correlation with skin diffusing activity.

Authors:  D McClean
Journal:  Biochem J       Date:  1943-07       Impact factor: 3.857

5.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

6.  Zone electrophoresis in a starch supporting medium.

Authors:  H G KUNKEL; R J SLATER
Journal:  Proc Soc Exp Biol Med       Date:  1952-05

7.  Staphylococcal hyaluronate lyase: purification and characterization studies.

Authors:  C Abramson; H Friedman
Journal:  J Bacteriol       Date:  1968-10       Impact factor: 3.490

8.  Staphylococcal hyaluronate lyase: multiple electrophoretic and chromatographic forms.

Authors:  C Abramson
Journal:  Arch Biochem Biophys       Date:  1967-07       Impact factor: 4.013

9.  Studies on extracellular PROTEINS FROM Staphylococcus aureus. I. Separation and characterization of enzymes and toxins by isoelectric focusing.

Authors:  O Vesterberg; T Wadström; K Vesterberg; H Svensson; B Malmgren
Journal:  Biochim Biophys Acta       Date:  1967-04-11
  9 in total
  1 in total

1.  Purification and properties of streptococcal hyaluronate lyase.

Authors:  J Hill
Journal:  Infect Immun       Date:  1976-09       Impact factor: 3.441
  1 in total

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