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Literature DB >> 4972781

Mechanisms of enhancement of SP82 transfection.

Abstract

SP82 transfection in Bacillus subtilis could be markedly increased by exposing the competent cells to ultraviolet (UV)-irradiated homologous or heterologous cellular deoxyribonucleic acid (DNA). This enhancement was similar in time and level of peak effect to enhancements effected by preinfection with helper phages or by UV irradiation of competent cells. The effectiveness of various DNA preparations in increasing transfection paralleled the adenine plus thymidine content of the preparations and was maximal at UV doses approaching those which were maximal for pyrimidine dimerization. The most probable interpretation is that irradiated DNA binds cellular nucleases which would otherwise inactivate the incoming transfecting DNA.

Entities: Chemical Species

Mesh：

Substances：
DNA

Year: 1968 PMID： 4972781 PMCID： PMC375678 DOI： 10.1128/JVI.2.7.710-715.1968

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

9 in total

13 in total

Review 1. Transfection of Enterobacteriaceae and its applications.

Authors: R Benzinger
Journal: Microbiol Rev Date: 1978-03

2. Factors affecting bacterial competence for transfection and transfection enhancement.

Authors: H T Epstein
Journal: Bacteriol Rev Date: 1968-12

Review 3. Bacteriophages of Bacillus subtilis.

Authors: H E Hemphill; H R Whiteley
Journal: Bacteriol Rev Date: 1975-09

4. Genetic transformation in Staphylococcus aureus: demonstration of a competence-conferring factor of bacteriophage origin in bacteriophage 80 alpha lysates.

Authors: N E Thompson; P A Pattee
Journal: J Bacteriol Date: 1981-10 Impact factor: 3.490

Mechanisms of enhancement of SP82 transfection.

1. The bacterial chromosome and its manner of replication as seen by autoradiography.

2. THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM.

3. INFECTIVITY OF DNA ISOLATED FROM BACILLUS SUBTILIS BACTERIOPHAGE, SP82.

4. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.

5. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.

6. Transfection enhancement by ultraviolet irradiation.

7. An estimate of the action spectrum for ultraviolet enhancement of transfection.

8. Comparison of ultraviolet sensitivity of Bacillus subtilis bacteriophage SPO2 and its infectious DNA.

9. Autoradiography of the Bacillus subtilis chromosome.

Review 1. Transfection of Enterobacteriaceae and its applications.

2. Factors affecting bacterial competence for transfection and transfection enhancement.

Review 3. Bacteriophages of Bacillus subtilis.

4. Genetic transformation in Staphylococcus aureus: demonstration of a competence-conferring factor of bacteriophage origin in bacteriophage 80 alpha lysates.

5. Bacteriophage SP82G inhibition of an intracellular deoxyribonucleic acid inactivation process in Bacillus subtilis.

6. X-ray study of competence development in Bacillus subtilis.

7. Source of the nonlinear dependence of bacteriophage SP82 transfection on deoxyribonucleic acid concentration.

8. DNA fixation and development of transformability and transfectability in Bacillus subtilis.

9. Recombinational-type transfer of viral DNA during bacteriophage 2C replication in Bacillus subtilis.

10. Effect of lysogeny on transfection and transfection enhancement in Bacillus subtilis.