Literature DB >> 4966474

Effect of glutathione on ribonuclease.

S Kim, W K Paik.   

Abstract

GSH, but not GSSG, inhibits the reactivation by phosphate ion of ribonuclease activity inactivated by urea or guanidine. The effects of GSH are rather slow and pretreatment of ribonuclease with urea is a requisite for the inhibitory action of GSH on enzyme reactivation. GSH is more effective in urea than in guanidine and its action is greatly enhanced by EDTA. An optimum pH of about 9.0 was found for the inhibitory effect of GSH. Titration of the thiol groups formed after inactivation of ribonuclease by GSH strongly suggests that the reduction of only one disulphide linkage is involved. The reduction of this bond is sufficient to completely abolish the enzymic activity.

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Year:  1968        PMID： 4966474      PMCID： PMC1198562          DOI： 10.1042/bj1060707

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  9 in total

1.  The relationship of disulfide bonds and activity in ribonuclease.

Authors:  H RESNICK; J R CARTER; G KALNITSKY
Journal:  J Biol Chem       Date:  1959-07       Impact factor: 5.157

2.  PURIFICATION AND PROPERTIES OF A MICROSOMAL ENZYME SYSTEM CATALYZING THE REACTIVATION OF REDUCED RIBONUCLEASE AND LYSOZYME.

Authors:  R F GOLDBERGER; C J EPSTEIN; C B ANFINSEN
Journal:  J Biol Chem       Date:  1964-05       Impact factor: 5.157

3.  Studies on the reduction and re-formation of protein disulfide bonds.

Authors:  C B ANFINSEN; E HABER
Journal:  J Biol Chem       Date:  1961-05       Impact factor: 5.157

4.  Regeneration of enzyme activity by air oxidation of reduced subtilisin-modified ribonuclease.

Authors:  E HABER; C B ANFINSEN
Journal:  J Biol Chem       Date:  1961-02       Impact factor: 5.157

5.  The reductive cleavage of disulfide bonds and its application to problems of protein structure.

Authors:  M SELA; F H WHITE; C B ANFINSEN
Journal:  Biochim Biophys Acta       Date:  1959-02

6.  The relation of structure to enzymatic activity in ribonuclease.

Authors:  G KALNITSKY; J P HUMMEL; H RESNICK; J R CARTER; L B BARNETT; C DIERKS
Journal:  Ann N Y Acad Sci       Date:  1959-09-04       Impact factor: 5.691

7.  Reductive cleavage of disulfide bridges in ribonuclease.

Authors:  M SELA; F H WHITE; C B ANFINSEN
Journal:  Science       Date:  1957-04-12       Impact factor: 47.728

8.  The correlation of ribonuclease activity with specific aspects of tertiary structure.

Authors:  M SELA; C B ANFINSEN; W F HARRINGTON
Journal:  Biochim Biophys Acta       Date:  1957-12

9.  Studies on the specificity and mechanism of action of hepatic glutathione-insulin transhydrogenase.

Authors:  H M Katzen; F Tietze
Journal:  J Biol Chem       Date:  1966-08-10       Impact factor: 5.157
  9 in total

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