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Literature DB >> 4966082

The oxidation of cyst(e)ine by mast-cell tumour P815 in culture.

Abstract

1. Mast-cell tumour P815 cells oxidize [(35)S]cyst(e)ine to (35)SO(4) (2-). 2. Addition of cysteinesulphinate or sulphite decreases the formation of (35)SO(4) (2-); at the same time [(35)S]cysteinesulphinate or (35)SO(3) (2-) accumulates. 3. Extracts of P815 cells form sulphite from cysteinesulphinate and 2-oxoglutarate. The K(m) for cysteinesulphinate is 6.35mm and that for 2-oxoglutarate is 0.165mm. 4. Extracts oxidize sulphite to sulphate. 5. No formation of hydrogen sulphide from cyst(e)ine was detectable. 6. It is concluded that P815 cells oxidize cyst(e)ine to sulphate solely via cysteinesulphinate and sulphite. 7. The concentration of the enzymes catalysing this sequence is unaltered by several variations in the conditions of growth.

Entities: Chemical Disease

Mesh：

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Year: 1968 PMID： 4966082 PMCID： PMC1198521 DOI： 10.1042/bj1060437

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

13 in total

Review 1. DEVELOPMENT, MAINTENANCE AND ASSAY OF DRUG RESISTANCE.

Authors: G A FISCHER; A C SARTORELLI
Journal: Methods Med Res Date: 1964

2. Biosynthetic pathways in mastocytoma cells in culture and in vivo.

Authors: J P GREEN; M DAY
Journal: Ann N Y Acad Sci Date: 1963-02-26 Impact factor: 5.691

3. Enzymatic sulfation of mucopolysaccharides in hen oviduct. I. Transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to mucopolysaccharides.

Authors: S SUZUKI; J L STROMINGER
Journal: J Biol Chem Date: 1960-02 Impact factor: 5.157

4. A transplantable mast-cell neoplasm in the mouse.

Authors: T B DUNN; M POTTER
Journal: J Natl Cancer Inst Date: 1957-04 Impact factor: 13.506

5. Intermediary metabolism of L-cysteinesulfinic acid in animal tissues.

Authors: T P SINGER; E B KEARNEY
Journal: Arch Biochem Biophys Date: 1956-04 Impact factor: 4.013

6. The metabolism of i-cystine in the rat.

Authors: N R Lawrie
Journal: Biochem J Date: 1932 Impact factor: 3.857

7. Studies in the sulphur metabolism of the dog: The metabolism of glutathione compared with that of other cystine derivatives.

Authors: T S Hele; N W Pirie
Journal: Biochem J Date: 1931 Impact factor: 3.857

5. Vitamin A and the biosynthesis of sulphated mucopolysaccharides. Experiments with rats and cultured neoplastic mast cells.

Authors: D B Thomas; C A Pasternak
Journal: Biochem J Date: 1969-02 Impact factor: 3.857

5 in total

The oxidation of cyst(e)ine by mast-cell tumour P815 in culture.

Review 1. DEVELOPMENT, MAINTENANCE AND ASSAY OF DRUG RESISTANCE.

2. Biosynthetic pathways in mastocytoma cells in culture and in vivo.

3. Enzymatic sulfation of mucopolysaccharides in hen oviduct. I. Transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to mucopolysaccharides.

4. A transplantable mast-cell neoplasm in the mouse.

5. Intermediary metabolism of L-cysteinesulfinic acid in animal tissues.

6. The metabolism of i-cystine in the rat.

7. Studies in the sulphur metabolism of the dog: The metabolism of glutathione compared with that of other cystine derivatives.

8. The formation of sulphate from cysteine and methionine by tissues in vitro.

9. Transsulfuration in mammals. Microassays and tissue distributions of three enzymes of the pathway.

10. The synthesis of homocystine, cystathionine, and cystine by cultured diploid and heteroploid human cells.

1. Biosynthesis of glycosaminoglycans by cultured mastocytoma cells.

2. Turnover of mammalian phospholipids. Stable and unstable components in neoplastic mast cells.

3. The presence of glycopeptide material in cultured mouse mast-cell tumours.

Review 4. Molecular mechanisms and clinical implications of reversible protein S-glutathionylation.

5. Vitamin A and the biosynthesis of sulphated mucopolysaccharides. Experiments with rats and cultured neoplastic mast cells.