Literature DB >> 4962285

Improved assay method for phospholipase C.

S Kurioka, P V Liu.   

Abstract

A lecithin sol dispersed with deoxycholate was found to be attacked by phospholipase C in the presence of calcium ion more rapidly than were any other lecithin sols. The inorganic phosphate could be released quantitatively from the acid soluble phosphate liberated from lecithin by an excess amount of alkaline phosphatase present in phospholipase C reaction mixture. A simple and accurate assay method for phospholipase C was developed with the sol and the alkaline phosphatase.

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Year:  1967        PMID： 4962285      PMCID： PMC546967          DOI： 10.1128/am.15.3.551-555.1967

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  6 in total

1.  Phospholipase C determination by egg yolk turbidimetry.

Authors:  A C OTTOLENGHI
Journal:  Anal Biochem       Date:  1963-01       Impact factor: 3.365

2.  Electrokinetic requirements for the reaction between Cl. perfringens alpha-toxin (phospholipase C) and phospholipid substrates.

Authors:  A D BANGHAM; R M DAWSON
Journal:  Biochim Biophys Acta       Date:  1962-05-07

3.  Effects of solvents and surface-active agents on plastid phosphatidase C activity.

Authors:  M KATES
Journal:  Can J Biochem Physiol       Date:  1957-02

4.  The biochemistry of bacterial toxins: The lecithinase activity of Cl. welchii toxins.

Authors:  M G Macfarlane; B C Knight
Journal:  Biochem J       Date:  1941-09       Impact factor: 3.857

5.  The roles of various fractions of Pseudomonas aeruginosa in its pathogenesis. II. Effects of lecithinase and protease.

Authors:  P V Liu
Journal:  J Infect Dis       Date:  1966-02       Impact factor: 5.226

6.  Enzymic hydrolysis of sphingomyelin by rat liver.

Authors:  M Heller; B Shapiro
Journal:  Biochem J       Date:  1966-03       Impact factor: 3.857
  6 in total
  9 in total

1.  Identification of a new phospholipase C activity by analysis of an insertional mutation in the hemolytic phospholipase C structural gene of Pseudomonas aeruginosa.

Authors:  R M Ostroff; M L Vasil
Journal:  J Bacteriol       Date:  1987-10       Impact factor: 3.490

2.  Studies of phospholipase C (heat-labile hemolysin) in Pseudomonas aeruginosa.

Authors:  R M Berka; G L Gray; M L Vasil
Journal:  Infect Immun       Date:  1981-12       Impact factor: 3.441

3.  Purification and characterization of a Serratia marcescens metalloprotease.

Authors:  D Lyerly; A Kreger
Journal:  Infect Immun       Date:  1979-05       Impact factor: 3.441

4.  Secretion of phospholipase C by Pseudomonas aeruginosa.

Authors:  M W Stinson; C Hayden
Journal:  Infect Immun       Date:  1979-08       Impact factor: 3.441

5.  Cloning, sequencing, and role in virulence of two phospholipases (A1 and C) from mesophilic Aeromonas sp. serogroup O:34.

Authors:  S Merino; A Aguilar; M M Nogueras; M Regue; S Swift; J M Tomás
Journal:  Infect Immun       Date:  1999-08       Impact factor: 3.441

6.  Thin-layer chromatographic technique for rapid detection of bacterial phospholipases.

Authors:  N J Legakis; J Papavassiliou
Journal:  J Clin Microbiol       Date:  1975-11       Impact factor: 5.948

7.  Phospholipase C (heat-labile hemolysin) of Pseudomonas aeruginosa: purification and preliminary characterization.

Authors:  R M Berka; M L Vasil
Journal:  J Bacteriol       Date:  1982-10       Impact factor: 3.490

8.  Purification of Pseudomonas aeruginosa proteases and microscopic characterization of pseudomonal protease-induced rabbit corneal damage.

Authors:  A S Kreger; L D Gray
Journal:  Infect Immun       Date:  1978-02       Impact factor: 3.441

9.  Physicochemical fractionation of extracellular cornea-damaging proteases of Pseudomonas aeruginosa.

Authors:  A S Kreger; O K Griffin
Journal:  Infect Immun       Date:  1974-05       Impact factor: 3.441
  9 in total

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