Paper chromatographic system for the identification of glycerol in bacterial cell walls.

Ikawa, Miyoshi (University of New Hampshire, Durham), James W. Morrow, and Sheila J. Harney. Paper chromatographic system for the identification of glycerol in bacterial cell walls. J. Bacteriol. 92:812-814. 1966.-The solvent system consisting of isopropanol-5% boric acid (7:1, v/v) separates glycerol from the other carbohydrate constituents which are found in hydrolysates of bacterial cell walls. This system is useful for the identification of glycerol even when anhydroribitol and rhamnose are both present, and has been found to be applicable on cell wall hydrolysates as well as on synthetic mixtures.