Literature DB >> 4949505

In vitro attachment of radioactive endotoxins to lysosomes.

C Bona, L Chedid, A Lamensans.   

Abstract

The experiments reported here demonstrate that under certain conditions endotoxin can interact with lysosomes in vitro. After incubation of large granular fraction with (51)Cr-labeled antigen under the conditions required for acid hydrolytic activity, radioactivity was associated with the pellet after centrifugation. This effect can be inhibited by preincubation of the large granular fraction with unlabeled homologous or heterologous endotoxins. High resolution autoradiography showed that (14)C-labeled endotoxin was predominantly attached to the lysosomes contained in the large granular fraction. The mechanism of this interaction and its relationship to the toxic effect of endotoxins on lysosomes are discussed.

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Year:  1971        PMID： 4949505      PMCID： PMC416347          DOI： 10.1128/iai.4.5.532-536.1971

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  17 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Immunogenicity of lysosomal fractions prepared from leucocytes which pinocytized endotoxin.

Authors:  I Mesrobeanu; L Mesrobeanu; C Bona; D Vranialici; A Petrovici
Journal:  Z Immunitatsforsch Allerg Klin Immunol       Date:  1970-04

Review 3.  The role of lysosomes in the action of drugs and hormones.

Authors:  A Allison
Journal:  Adv Chemother       Date:  1968

4.  Localization and fate of 51-Cr-labeled somatic antigens of smooth and rough Salmonellae.

Authors:  L Chedid; F Parant; M Parant; F Boyer
Journal:  Ann N Y Acad Sci       Date:  1966-06-30       Impact factor: 5.691

Review 5.  Lysosome.

Authors:  G Weissmann
Journal:  N Engl J Med       Date:  1965-11-11       Impact factor: 91.245

6.  Reassociation of purified lipopolysaccharide and phospholipid of the bacterial cell envelope: electron microscopic and monolayer studies.

Authors:  L Rothfield; R W Horne
Journal:  J Bacteriol       Date:  1967-05       Impact factor: 3.490

7.  The subcellular distribution of antigen in macrophages.

Authors:  E Kölsch; N A Mitchison
Journal:  J Exp Med       Date:  1968-11-01       Impact factor: 14.307

8.  Improvements in epoxy resin embedding methods.

Authors:  J H LUFT
Journal:  J Biophys Biochem Cytol       Date:  1961-02

9.  Lysosomes in lymphoid tissue. I. The measurement of hydrolytic activities in whole homogenates.

Authors:  W E Bowers; J T Finkenstaedt; C de Duve
Journal:  J Cell Biol       Date:  1967-02       Impact factor: 10.539

10.  Antigens in immunity. XIV. Electron microscopic radioautographic studies of antigen capture in the lymph node medulla.

Authors:  G J Nossal; A Abbot; J Mitchell
Journal:  J Exp Med       Date:  1968-02-01       Impact factor: 14.307
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  3 in total

1.  Endotoxin-tobramycin additive toxicity on renal proximal tubular cells in culture.

Authors:  V Joly; Y Bergeron; M G Bergeron; C Carbon
Journal:  Antimicrob Agents Chemother       Date:  1991-02       Impact factor: 5.191

2.  Influence of endotoxin on the intrarenal distribution of gentamicin, netilmicin, tobramycin, amikacin, and cephalothin.

Authors:  M G Bergeron; Y Bergeron
Journal:  Antimicrob Agents Chemother       Date:  1986-01       Impact factor: 5.191

3.  Autoradiography of tobramycin uptake by the proximal and distal tubules of normal and endotoxin-treated rats.

Authors:  M G Bergeron; Y Bergeron; Y Marois
Journal:  Antimicrob Agents Chemother       Date:  1986-06       Impact factor: 5.191
  3 in total

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