Literature DB >> 4943554

A complex between initiation factor IF2, guanosine triphosphate, and fMet-tRNA: an intermediate in initiation complex formation.

A H Lockwood, P R Chakraborty, U Maitra.   

Abstract

Evidence is presented that suggests the formation of a complex between polypeptide chain-initiation factor IF 2, GTP, and fMet-tRNA(f). This complex transfers both fMet-tRNA(f) and GTP to 30S ribosomal subunits in the presence of ApUpG and initiation factor IF 1. The resultant 30S initiation complex reacts with 50S subunits to form a 70S initiation complex. fMet-tRNA(f) in this 70S complex reacts with puromycin to form fMet-puromycin. These results suggest that [IF 2, GTP, fMet-tRNA(f)] is an intermediate in the initiation of protein synthesis in Escherichia coli.

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Year:  1971        PMID： 4943554      PMCID： PMC389604          DOI： 10.1073/pnas.68.12.3122

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  16 in total

1.  Protein factors involved in polypeptide chain initiation in Escherichia coli.

Authors:  J S Dubnoff; U Maitra
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1969

2.  Purification and properties of initiation factors F1 and F2.

Authors:  R E Thach; J W Hershey; D Kolakofsky; K F Dewey; E Remold-O'Donnell
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1969

3.  Stability of different ribosomal complexes with initiator transfer RNA and synthetic messenger RNA.

Authors:  M Grunberg-Manago; B F Clark; M Revel; P S Rudland; J Dondon
Journal:  J Mol Biol       Date:  1969-02-28       Impact factor: 5.469

4.  Translation of the genetic message. VII. Role of initiation factors in formation of the chain initiation complex with Escherichia coli ribosomes.

Authors:  K Iwasaki; S Sabol; A J Wahba; S Ochoa
Journal:  Arch Biochem Biophys       Date:  1968-05       Impact factor: 4.013

5.  Function of three protein factors and ribosomal subunits in the initiation of protein synthesis in E. coli.

Authors:  M Revel; J C Lelong; G Brawerman; F Gros
Journal:  Nature       Date:  1968-09-07       Impact factor: 49.962

6.  Junction of the 50S ribosomal subunit with the 30S initiation complex.

Authors:  D Kolakofsky; T Ohta; R E Thach
Journal:  Nature       Date:  1968-10-19       Impact factor: 49.962

7.  Initiation of protein synthesis II. A convenient assay for the ribosome-dependent synthesis of N-formyl-C14-methionylpuromycin.

Authors:  P Leder; H Bursztyn
Journal:  Biochem Biophys Res Commun       Date:  1966-10-20       Impact factor: 3.575

8.  Guanosine 5'-triphosphatase activity of initiation factor f2.

Authors:  D Kolakofsky; K F Dewey; J W Hershey; R E Thach
Journal:  Proc Natl Acad Sci U S A       Date:  1968-11       Impact factor: 11.205

9.  Polypeptide chain initiation in E. coli: sulfhydryl groups and the function of initiation factor F2.

Authors:  R Mazumder; Y B Chae; S Ochoa
Journal:  Proc Natl Acad Sci U S A       Date:  1969-05       Impact factor: 11.205

10.  RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES.

Authors:  M NIRENBERG; P LEDER
Journal:  Science       Date:  1964-09-25       Impact factor: 47.728
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  13 in total

Review 1.  Initiation of protein synthesis in bacteria.

Authors:  Brian Søgaard Laursen; Hans Peter Sørensen; Kim Kusk Mortensen; Hans Uffe Sperling-Petersen
Journal:  Microbiol Mol Biol Rev       Date:  2005-03       Impact factor: 11.056

2.  Initiation factor 2 crystal structure reveals a different domain organization from eukaryotic initiation factor 5B and mechanism among translational GTPases.

Authors:  Daniel Eiler; Jinzhong Lin; Angelita Simonetti; Bruno P Klaholz; Thomas A Steitz
Journal:  Proc Natl Acad Sci U S A       Date:  2013-09-12       Impact factor: 11.205

Review 3.  Antibiotics as probes of biological complexity.

Authors:  Shannon B Falconer; Tomasz L Czarny; Eric D Brown
Journal:  Nat Chem Biol       Date:  2011-07       Impact factor: 15.040

4.  Interchangeability of factors and tRNA's in bacterial and eukaryotic translation initiation systems.

Authors:  F Berthelot; D Bogdanovsky; G Schapira; F Gros
Journal:  Mol Cell Biochem       Date:  1973-05-11       Impact factor: 3.396

5.  Factor-promoted dissociation of free ribosomes in a rabbit reticulocyte lysate system: inhibition and requirement for an energy source.

Authors:  S Mizuno; M Rabinovitz
Journal:  Proc Natl Acad Sci U S A       Date:  1973-03       Impact factor: 11.205

6.  Protein initiation in eukaryotes: formation and function of a ternary complex composed of a partially purified ribosomal factor, methionyl transfer RNA, and guanosine triphosphate.

Authors:  D H Levin; D Kyner; G Acs
Journal:  Proc Natl Acad Sci U S A       Date:  1973-01       Impact factor: 11.205

7.  Specific in situ cleavage of 16S ribosomal RNA of Escherichia coli interferes with the function of initiation factor IF-1.

Authors:  R A Baan; J J Duijfjes; E van Leerdam; P H van Knippenberg; L Bosch
Journal:  Proc Natl Acad Sci U S A       Date:  1976-03       Impact factor: 11.205

8.  Initiation factor 2-dependent ribosomal binding of N-formylmethionyl-transfer RNA without added guanosine triphosphate.

Authors:  R Mazumder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-10       Impact factor: 11.205

9.  Lack of inhibition by L-1-chloro-4-phenyl-3-toluene-p-sulphonamidobutan-2-one (L-1-tosylamido-2-phenylethyl chloromethyl ketone) of the formation of bacterial polypeptide chain initiation complex.

Authors:  J Jonák; B F Clark
Journal:  Biochem J       Date:  1973-10       Impact factor: 3.857

Review 10.  Single-molecule analysis of translational dynamics.

Authors:  Alexey Petrov; Jin Chen; Seán O'Leary; Albert Tsai; Joseph D Puglisi
Journal:  Cold Spring Harb Perspect Biol       Date:  2012-09-01       Impact factor: 10.005
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