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Literature DB >> 4943549

New polarity suppressors in Escherichia coli: suppression and messenger RNA stability.

Abstract

Two new polarity suppressors (Su27 and Su78) have been isolated in Escherichia coli. Both suppress polarity in the trp and lac operons, and neither shows codon specificity for suppression. Extreme polar mutants of the lac z gene that contain either Su27 or Su78 make mRNA from the entire operon; however, no active beta-galactosidase is translated from the z-gene messenger, and the amount of distal messenger is greater than would be expected from corresponding enzyme activities. Puromycin treatment of lac(+) strains mimics the effect of polar chain-termination mutations in destabilizing lac mRNA. This effect is completely reversed by Su27, and it is partially reversed by Su78. The results suggest that these suppressors act by stabilizing mRNA after premature termination of protein synthesis.

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Year: 1971 PMID： 4943549 PMCID： PMC389570 DOI： 10.1073/pnas.68.12.2962

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

26 in total

1. A SECOND PERMEASE FOR METHYL-THIO-BETA-D-GALACTOSIDE IN ESCHERICHIA COLI.

Authors: L S PRESTIDGE; A B PARDEE
Journal: Biochim Biophys Acta Date: 1965-05-04

2. The periodicity of RNA polymerase initiations: a new regulatory feature of transcription.

Authors: R F Baker; C Yanofsky
Journal: Proc Natl Acad Sci U S A Date: 1968-05 Impact factor: 11.205

3. Mutational alteration of the maximal level of Lac operon expression.

Authors: J Scaife; J R Beckwith
Journal: Cold Spring Harb Symp Quant Biol Date: 1966

4. Polarity in the tryptophan operon of E. coli.

Authors: F Imamoto; J Ito; C Yanofsky
Journal: Cold Spring Harb Symp Quant Biol Date: 1966

5. Effect of nonsense mutations on translation of the lactose operon of Escherichia coli.

Authors: A Newton
Journal: Cold Spring Harb Symp Quant Biol Date: 1966

6. The formation and stabilization of 30S and 50S ribosome couples in Escherichia coli.

Authors: D Schlessinger; G Mangiarotti; D Apirion
Journal: Proc Natl Acad Sci U S A Date: 1967-10 Impact factor: 11.205

7. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.

Authors: D Gillespie; S Spiegelman
Journal: J Mol Biol Date: 1965-07 Impact factor: 5.469

8. Re-initiation of polypeptide synthesis and polarity in the lac operon of Escherichia coli.

Authors: A Newton
Journal: J Mol Biol Date: 1969-05-14 Impact factor: 5.469

9. Messenger RNA stability and polarity in the Lac operon of Escherichia coli.

Authors: T Carter; A Newton
Journal: Nature Date: 1969-08-16 Impact factor: 49.962

10. Increased stability of polysomes in an Escherichia coli mutant with relaxed control of RNA synthesis.

Authors: E Z Ron; R E Kohler; B D Davis
Journal: Proc Natl Acad Sci U S A Date: 1966-08 Impact factor: 11.205

27 in total

1. Transcription and membrane attachment of bacteriophage lambda DNA in the absence of N function in the E. coli suA 1 mutant.

Authors: M F Bourguignon-Van Horen; N Henry-Van der Loo
Journal: Mol Gen Genet Date: 1979-11

10. Efficient suppression of the requirement for N function of bacteriophage lambda by a Rho-defective E.coli suA mutant.

Authors: M Belfort; A B Oppenheim
Journal: Mol Gen Genet Date: 1976-10-18

New polarity suppressors in Escherichia coli: suppression and messenger RNA stability.

1. A SECOND PERMEASE FOR METHYL-THIO-BETA-D-GALACTOSIDE IN ESCHERICHIA COLI.

2. The periodicity of RNA polymerase initiations: a new regulatory feature of transcription.

3. Mutational alteration of the maximal level of Lac operon expression.

4. Polarity in the tryptophan operon of E. coli.

5. Effect of nonsense mutations on translation of the lactose operon of Escherichia coli.

6. The formation and stabilization of 30S and 50S ribosome couples in Escherichia coli.

7. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.

8. Re-initiation of polypeptide synthesis and polarity in the lac operon of Escherichia coli.

9. Messenger RNA stability and polarity in the Lac operon of Escherichia coli.

10. Increased stability of polysomes in an Escherichia coli mutant with relaxed control of RNA synthesis.

1. Transcription and membrane attachment of bacteriophage lambda DNA in the absence of N function in the E. coli suA 1 mutant.

2. The Genetic Basis for Mucoidy and Radiation Sensitivity in capR (lon) Mutants of E. coli K-12.

3. Suppression of polarity of insertion mutations in the gal operon and N mutations in bacteriophage lambda.

4. Hyperdegradation of proteins in Escherichia coli rho mutants.

5. Bacteria with defective rho factors suppress the effects of N mutations in bacteriophage lambda.

6. Polypeptide formation and polyribosomes in Escherichia coli treated with chloramphenicol.

7. Salmonella typhimurium mutants lacking ribonuclease I: effect on the polarity of histidine mutants.

8. Catabolite translational effects on the lac messenger RNA of Escherichia coli K12.

9. Specificity of polarity suppression in E. coli: correction of defects in gene N, but not in gene Q, of phage lambda.

10. Efficient suppression of the requirement for N function of bacteriophage lambda by a Rho-defective E.coli suA mutant.