Literature DB >> 4937781

New method for extraction of ribonucleic acid and polyribosomes from Schizosaccharomyces pombe.

H C Birnboim.   

Abstract

A new method for extracting ribosomal ribonucleic acid (RNA) and polyribosomes from Schizosaccharomyces pombe, a fission yeast, has been developed. Cells grown in the presence of 2-deoxyglucose were found to be more sensitive to the action of Glusulase; a short period of treatment (10 to 20 min) at 0 C was sufficient to convert the cells to osmotically sensitive structures. From these, ribosomal RNA and polyribosomes were readily prepared.

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Year:  1971        PMID： 4937781      PMCID： PMC246985          DOI： 10.1128/jb.107.3.659-663.1971

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  13 in total

Review 1.  Ribonucleic acids from animal cells.

Authors:  J E Darnell
Journal:  Bacteriol Rev       Date:  1968-09

2.  Temperature-sensitive mutants of yeast exhibiting a rapid inhibition of protein synthesis.

Authors:  L H Hartwell; C S McLaughlin
Journal:  J Bacteriol       Date:  1968-11       Impact factor: 3.490

3.  The synthesis and processing of ribosomal RNA precursor molecules in yeast.

Authors:  R L Taber; W S Vincent
Journal:  Biochim Biophys Acta       Date:  1969-08-20

4.  Ribosomal precursor RNA in Saccharomyces carlsbergensis.

Authors:  J Retèl; R J Planta
Journal:  Eur J Biochem       Date:  1967-12

5.  RNA metabolism in the HeLa cell nucleus.

Authors:  S Penman
Journal:  J Mol Biol       Date:  1966-05       Impact factor: 5.469

6.  Lysis of yeast cell walls induced by 2-deoxyglucose at their sites of glucan synthesis.

Authors:  B F Johnson
Journal:  J Bacteriol       Date:  1968-03       Impact factor: 3.490

7.  Macromolecule synthesis in yeast spheroplasts.

Authors:  H T Hutchison; L H Hartwell
Journal:  J Bacteriol       Date:  1967-11       Impact factor: 3.490

8.  Macromolecule synthesis in temperature-sensitive mutants of yeast.

Authors:  L H Hartwell
Journal:  J Bacteriol       Date:  1967-05       Impact factor: 3.490

9.  Ribonucleic acid synthesis in yeast. The effect of cycloheximide on the synthesis of ribonucleic acid in Saccharomyces carlsbergensis.

Authors:  S R de Kloet
Journal:  Biochem J       Date:  1966-06       Impact factor: 3.857

10.  Effect of 2-deoxyglucose on Schizosaccharomyces pombe.

Authors:  R Megnet
Journal:  J Bacteriol       Date:  1965-10       Impact factor: 3.490
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  5 in total

1.  Excision of pyrimidine dimers by several UV-sensitive mutants of S. pombe.

Authors:  H C Birnboim; A Nasim
Journal:  Mol Gen Genet       Date:  1975

2.  Detection of pyrimidine dimers in hydrolysates of yeast DNA by high voltage paper electrophoresis.

Authors:  H C Birnboim; A Nassim
Journal:  Mol Gen Genet       Date:  1974-06-27

3.  Recessive super-suppression in yeast.

Authors:  V N Smirnov; V G Kreier; L V Lizlova; V M Andrianova; S G Inge-Vechtomov
Journal:  Mol Gen Genet       Date:  1974-03-14

4.  Effect of 2-deoxy-D-glucose on growth and cell walls of Schizosaccharomyces pombe 972h.

Authors:  R K Poole; D Lloyd
Journal:  Arch Mikrobiol       Date:  1973

5.  Morphogenic effect of 2-deoxy-D-arabino-hexose on Rhodosporidium toruloides.

Authors:  M Sipiczki; V Farkas
Journal:  Folia Microbiol (Praha)       Date:  1979       Impact factor: 2.099
  5 in total

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