Literature DB >> 4928818

Purification and physical properties of group C streptococcal phage-associated lysin.

V A Fischetti, E C Gotschlich, A W Bernheimer.   

Abstract

A purification procedure for the Group C phage-associated lysin is described utilizing tetrathionate to protect the enzyme's -SH group(s) from thiol-inactivating agents. A 652-fold purification has been accomplished yielding a solution in which the enzyme activity corresponds to essentially a single band on polyacrylamide gel which accounts for 70% of the total protein in the preparation. A molecular weight of 101,000 and frictional ratio of 1.526 was determined for the lysin utilizing experimentally determined values for its Stokes radius and sedimentation coefficient.

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Year:  1971        PMID： 4928818      PMCID： PMC2138921          DOI： 10.1084/jem.133.5.1105

Source DB:  PubMed          Journal:  J Exp Med        ISSN： 0022-1007            Impact factor:   14.307


  30 in total

1.  MOLECULAR EXCLUSION AND RESTRICTED DIFFUSION PROCESSES IN MOLECULAR-SIEVE CHROMATOGRAPHY.

Authors:  G K ACKERS
Journal:  Biochemistry       Date:  1964-05       Impact factor: 3.162

2.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

3.  Studies on the bacteriophages of hemolytic streptococci. II. Antigens released from the streptococcal cell wall by a phage-associated lysin.

Authors:  R M KRAUSE
Journal:  J Exp Med       Date:  1958-12-01       Impact factor: 14.307

4.  A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

Authors:  R G MARTIN; B N AMES
Journal:  J Biol Chem       Date:  1961-05       Impact factor: 5.157

5.  Enzymic Activity of Bacteriophage-Culture Lysates: I. A Capsule Lysin Active against Klebsiella pneumoniae Type A.

Authors:  J C Humphries
Journal:  J Bacteriol       Date:  1948-11       Impact factor: 3.490

6.  Biosynthesis of streptococcal cell walls: A rhamnose polysaccharide.

Authors:  L D ZELEZNICK; J J BOLTRALIK; S S BARKULIS; C SMITH; H HEYMANN
Journal:  Science       Date:  1963-04-26       Impact factor: 47.728

7.  INTRACELLULAR M PROTEIN OF GROUP A STREPTOCOCCUS.

Authors:  E N FOX
Journal:  J Bacteriol       Date:  1963-03       Impact factor: 3.490

8.  OBSERVATIONS ON THE GROUP C STREPTOCOCCAL BACTERIOPHAGE AND LYTIC ENZYME SYSTEM.

Authors:  E N FOX; M K WITTNER
Journal:  J Bacteriol       Date:  1965-02       Impact factor: 3.490

9.  An immunological relationship between the group. A streptococcus and mammalian muscle.

Authors:  J B Zabriskie; E H Freimer
Journal:  J Exp Med       Date:  1966-10-01       Impact factor: 14.307

10.  Studies on L forms and protoplasts of group A streptococci. II. Chemical and immunological properties of the cell membrane.

Authors:  E H FREIMER
Journal:  J Exp Med       Date:  1963-03-01       Impact factor: 14.307
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  44 in total

1.  A novel sortase, SrtC2, from Streptococcus pyogenes anchors a surface protein containing a QVPTGV motif to the cell wall.

Authors:  Timothy C Barnett; Aman R Patel; June R Scott
Journal:  J Bacteriol       Date:  2004-09       Impact factor: 3.490

2.  Chemical analysis of changes in membrane composition during growth of Streptococcus pyogenes.

Authors:  I van de Rijn; R E Kessler
Journal:  Infect Immun       Date:  1979-12       Impact factor: 3.441

3.  Purification and properties of streptococcal nicotinamide adenine dinucleotide glycohydrolase.

Authors:  P S Grushoff; S Shany; A W Bernheimer
Journal:  J Bacteriol       Date:  1975-05       Impact factor: 3.490

4.  PlyC: a multimeric bacteriophage lysin.

Authors:  Daniel Nelson; Raymond Schuch; Peter Chahales; Shiwei Zhu; Vincent A Fischetti
Journal:  Proc Natl Acad Sci U S A       Date:  2006-07-03       Impact factor: 11.205

Review 5.  Phage lytic enzymes: a history.

Authors:  David Trudil
Journal:  Virol Sin       Date:  2015-02-05       Impact factor: 4.327

6.  Turning a new phage.

Authors:  Lauren Gravitz
Journal:  Nat Med       Date:  2012-09       Impact factor: 53.440

7.  Streptococcus pyogenes type 12 M protein gene regulation by upstream sequences.

Authors:  J C Robbins; J G Spanier; S J Jones; W J Simpson; P P Cleary
Journal:  J Bacteriol       Date:  1987-12       Impact factor: 3.490

Review 8.  Streptococcal M protein: molecular design and biological behavior.

Authors:  V A Fischetti
Journal:  Clin Microbiol Rev       Date:  1989-07       Impact factor: 26.132

9.  Streptococcin A-FF22: nisin-like antibiotic substance produced by a group A streptococcus.

Authors:  J R Tagg; L W Wannamaker
Journal:  Antimicrob Agents Chemother       Date:  1978-07       Impact factor: 5.191

10.  Simple procedure for production by group C streptococci of phage-associated lysin active against group A streptococci.

Authors:  J O Cohen; H Gross; W K Harrell
Journal:  Appl Microbiol       Date:  1975-02
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