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Literature DB >> 4926681

Influence of thymine starvation on the integrity of deoxyribonucleic acid in Escherichia coli.

Abstract

The influence of thymine starvation on the single-strand molecular weight of deoxyribonucleic acid (DNA) from Escherichia coli was determined by sedimentation through gradients of alkaline sucrose. Growth of cells for as long as 150 min in thymineless medium did not significantly reduce the molecular weight below the control value of 2.4 +/- 0.3 x 10(8) daltons. Incubation of cells in thymineless medium after exposure to 500 ergs/mm(2) of ultraviolet light or 20 krad of (137)Cs gamma rays did not appear to block the rejoining of single-strand breaks associated with irradiation. Thus, DNA repair enzymes, presumably including DNA ligase, are not significantly inhibited by thymine starvation.

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Year: 1971 PMID： 4926681 PMCID： PMC248494 DOI： 10.1128/jb.105.3.733-738.1971

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

18 in total

1. The bacterial chromosome and its manner of replication as seen by autoradiography.

Authors: J CAIRNS
Journal: J Mol Biol Date: 1963-03 Impact factor: 5.469

2. Thymineless induction in Escherichia coli K12 (lambda).

Authors: D KORN; A WEISSBACH
Journal: Biochim Biophys Acta Date: 1962-11-26

3. Morphological changes in Escherichia coli strain C produced by treatments affecting deoxyribonucleic acid synthesis.

Authors: J C Suit; T Barbee; S Jetton
Journal: J Gen Microbiol Date: 1967-10

4. Radiation-induced reorientation of chromosome replication sequence: generality in Escherichia coli; independence of prophage or 5-bromouracil toxicity.

Authors: R Hewitt; D Billen; G Jorgensen
Journal: Radiat Res Date: 1967-10 Impact factor: 2.841

4. Influence of thymine starvation on the integrity of episomal and chromosomal deoxyribonucleic acids in Escherichia coli CR34 (lambda ind-).

Authors: R C Shuster
Journal: J Bacteriol Date: 1973-11 Impact factor: 3.490

8. Single-strand breaks in deoxyribonucleic acid and viability loss during deoxyribonucleic acid synthesis inhibition in Escherichia coli.

Authors: W E Hill; W L Fangman
Journal: J Bacteriol Date: 1973-12 Impact factor: 3.490

9. Thymineless death and ultraviolet sensitivity in Micrococcus radiodurans.

Authors: J G Little; P C Hanawalt
Journal: J Bacteriol Date: 1973-01 Impact factor: 3.490

10. Genetic damage during thymidylate starvation in Saccharomyces cerevisiae.

Authors: B J Barclay; J G Little
Journal: Mol Gen Genet Date: 1978-03-20

Influence of thymine starvation on the integrity of deoxyribonucleic acid in Escherichia coli.

1. The bacterial chromosome and its manner of replication as seen by autoradiography.

2. Thymineless induction in Escherichia coli K12 (lambda).

3. Morphological changes in Escherichia coli strain C produced by treatments affecting deoxyribonucleic acid synthesis.

4. Radiation-induced reorientation of chromosome replication sequence: generality in Escherichia coli; independence of prophage or 5-bromouracil toxicity.

5. Interruptions in single strands of the DNA in slime mold and other organisms.

6. Linkage of polynucleotides through phosphodiester bonds by an enzyme from Escherichia coli.

7. The excision of thymine dimer from the DNA of UV-irradiated E. coli 15 T-A-U during thymine deprivation.

8. Nonconservative DNA replication in bacteria after thymine starvation.

9. Thymineless death in Escherichia coli 15T- and recombinants of 15T- and Escherichia coli K-12.

10. Utilization of 5-bromouracil by thymineless bacteria.

1. Size and transforming activity of deoxyribonucleic acid in Diplococcus pneumoniae during thymidine starvation.

2. Curing of an R factor Escherichia coli by trimethoprim.

3. Loss of deoxyribonucleic acid-thymine during thymine starvation of Escherichia coli.

4. Influence of thymine starvation on the integrity of episomal and chromosomal deoxyribonucleic acids in Escherichia coli CR34 (lambda ind-).

5. Ultraviolet-sensitive mutator strain of Escherichia coli K-12.

6. Sedimentation analysis of deoxyribonucleic acid from thymine-starved Escherichia coli.

7. Thymineless death in Bacillus subtilis: correlation between cell lysis and deoxyribonucleic acid breakdown.

8. Single-strand breaks in deoxyribonucleic acid and viability loss during deoxyribonucleic acid synthesis inhibition in Escherichia coli.

9. Thymineless death and ultraviolet sensitivity in Micrococcus radiodurans.

10. Genetic damage during thymidylate starvation in Saccharomyces cerevisiae.