Literature DB >> 49216

Lactoglobulins (goat) in the immunofluorescence staining of parainfluenza virus type 2 in L-132 cells.

S A Sattar, C A Mitchell.   

Abstract

Instillation of parainfluenza virus type 2 in the mammary gland of the goat resulted in the appearance of specific and high titered antibodies in the milk. The globulin fraction of the milk was separated and conjugated with fluorescein isothiocyanate. These conjugated antibodies could be successfully used in the specific staining of the homologous virus in infected L-132 cells. Therefore, milk from such immunized animals represents a rich and convenient source of specific antibodies for use in immunofluorescence and other serological tests.

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Year:  1975        PMID: 49216      PMCID: PMC1277474     

Source DB:  PubMed          Journal:  Can J Comp Med        ISSN: 0008-4050


  8 in total

1.  Isothiocyanate compounds as fluorescent labeling agents for immune serum.

Authors:  J L RIGGS; R J SEIWALD; J H BURCKHALTER; C M DOWNS; T G METCALF
Journal:  Am J Pathol       Date:  1958 Nov-Dec       Impact factor: 4.307

2.  Antibody production in milk serum after virus instillation of goat mammary gland. I. Responses to influenza virus, mumps virus, and adenovirus 3.

Authors:  C A Mitchell; L F Guerin; A E Pasieka
Journal:  Can J Microbiol       Date:  1967-08       Impact factor: 2.419

3.  Plastic chambers for the cultivation of cells on microscope slides and their application in virology.

Authors:  S A Sattar; J C Westwood
Journal:  Can J Microbiol       Date:  1967-08       Impact factor: 2.419

4.  Fluorescein-labeled goat milk antibodies: use in detection of mumps virus in tissue culture.

Authors:  S A Sattar; C A Mitchell; L F Guerin
Journal:  Can J Comp Med Vet Sci       Date:  1967-10

5.  Dynamics of immunofluorescence and infectivity of parainfluenza 3 virus in susceptible cells.

Authors:  A A Sominina; Y N Zubzhitsky; A A Smorodintsev
Journal:  Acta Virol       Date:  1967-09       Impact factor: 1.162

6.  Antibody production in milk serum after virus instillation of goat mammary gland. IV. Experiments with protective properties of specific influenza antibody associated with lactogammaglobulin.

Authors:  G A Mitchell; L F Guerin; A E Pasieka
Journal:  Can J Comp Med       Date:  1971-01

7.  Antibody production in goat milk serum after virus instillation of goat mammary gland. V. Biochemical isolation and further characterization of antibodies to various influenza and mumps viruses.

Authors:  A E Pasieka; L F Guerin; C A Mitchell
Journal:  Can J Microbiol       Date:  1970-12       Impact factor: 2.419

8.  Antibody production in milk serum after virus instillation of goat mammary gland VI. "Sham infection" with special reference to rabies.

Authors:  C A Mitchell; L F Guerin; A E Pasieka
Journal:  Can J Comp Med       Date:  1974-01
  8 in total

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