Literature DB >> 4919990

Spermidine requirement for Bacillus thuringiensis ribosomes in cell-free phenylalanine incorporation.

L M Changchien, J N Aronson.   

Abstract

A cell-free system from Bacillus thuringiensis was found to actively incorporate phenylalanine into hot trichloroacetic acid-precipitable material in the presence of synthetic polynucleotide, ribosomes, S-100 supernatant, an energy-generating system, and guanosine triphosphate. Phenylalanine incorporation was absolutely dependent on the presence of spermidine in addition to magnesium ions, even when highly purified ribosomes were used. The spermidine effect could not be attributed to inhibition of nucleases. The ribosomal and supernatant fractions from Escherichia coli and B. thuringiensis could be substituted for each other in this system. The spermidine requirement was shown to be limited to the B. thuringiensis ribosome fraction.

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Year:  1970        PMID： 4919990      PMCID： PMC248151          DOI： 10.1128/jb.103.3.734-740.1970

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  35 in total

1.  LACK OF FIDELITY IN THE TRANSLATION OF SYNTHETIC POLYRIBONUCLEOTIDES.

Authors:  S M FRIEDMAN; I B WEINSTEIN
Journal:  Proc Natl Acad Sci U S A       Date:  1964-10       Impact factor: 11.205

2.  EFFECTS OF PUTRESCINE AND MAGNESIUM ON THE RIBOSOMES OF A PSEUDOMONAS.

Authors:  M L MOLLER; K KIM
Journal:  Biochem Biophys Res Commun       Date:  1965-06-18       Impact factor: 3.575

3.  Polyamines and ribosome structure.

Authors:  S S COHEN; J LICHTENSTEIN
Journal:  J Biol Chem       Date:  1960-07       Impact factor: 5.157

4.  Acetylornithinase of Escherichia coli: partial purification and some properties.

Authors:  H J VOGEL; D M BONNER
Journal:  J Biol Chem       Date:  1956-01       Impact factor: 5.157

5.  THE EFFECT OF POLYAMINES AND OF POLY U SIZE ON PHENYLALANINE INCORPORATION.

Authors:  R G Martin; B N Ames
Journal:  Proc Natl Acad Sci U S A       Date:  1962-12       Impact factor: 11.205

6.  Sucrose density-gradient analysis of Euglena gracilis RNA isolated with macaloid.

Authors:  K E Schuit; D E Buetow
Journal:  Biochim Biophys Acta       Date:  1968-10-29

7.  Biochemical studies of bacterial sporulation and germination. XI. Protein-synthesizing systems from vegetative cells and spores of Bacillus megaterium.

Authors:  M P Deutscher; P Chambon; A Konberg
Journal:  J Biol Chem       Date:  1968-10-10       Impact factor: 5.157

Review 8.  A critical review of Bacillus thuringiensis var. thuringiensis Berliner and other crystalliferous bacteria.

Authors:  A M Heimpel
Journal:  Annu Rev Entomol       Date:  1967       Impact factor: 19.686

9.  Polynucleotide-dependent incorporation of amino acids in a cell-free system from thermophilic bacteria.

Authors:  I D Algranati; P Lengyel
Journal:  J Biol Chem       Date:  1966-04-25       Impact factor: 5.157

10.  Polyamines and protein synthesis. II. The shift in optimal concentration of Mg2+ by polyamines in the MS2 phage RNA-directed polypeptide synthesis.

Authors:  Y Takeda
Journal:  Biochim Biophys Acta       Date:  1969-03-18
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  8 in total

Review 1.  Polyamines, ribonucleases, and the stability of RNA.

Authors:  T P Karpetsky; P A Hieter; J J Frank; C C Levy
Journal:  Mol Cell Biochem       Date:  1977-09-09       Impact factor: 3.396

2.  Regulation of macromolecular synthesis by putrescine in a conditional Escherichia coli putrescine auxotroph.

Authors:  D V Young; P R Srinivasan
Journal:  J Bacteriol       Date:  1972-10       Impact factor: 3.490

3.  Inhibition of cell-free protein synthesis by hydrostatic pressure.

Authors:  J R Schwarz; J V Landau
Journal:  J Bacteriol       Date:  1972-12       Impact factor: 3.490

4.  In vitro expression of a Tn9-derived chloramphenicol acetyltransferase gene fusion by using a Bacillus subtilis system.

Authors:  T I Zaghloul; R H Doi
Journal:  J Bacteriol       Date:  1987-03       Impact factor: 3.490

5.  Polyamine synthesis and accumulation in Escherichia coli infected with bacteriophage R17.

Authors:  I Fukuma; S S Cohen
Journal:  J Virol       Date:  1973-12       Impact factor: 5.103

6.  Polyamine levels during growth, sporulation, and spore germination of Bacillus megaterium.

Authors:  P Setlow
Journal:  J Bacteriol       Date:  1974-03       Impact factor: 3.490

7.  Heterotrophic nature of the cell-free protein-synthesizing system from the strict chemolithotroph, Thiobacillus thiooxidans.

Authors:  K Amemiya; W W Umbreit
Journal:  J Bacteriol       Date:  1974-02       Impact factor: 3.490

8.  Natural messenger ribonucleic acid-directed cell-free protein-synthesizing system of Bacillus subtilis.

Authors:  L Legault-Demare; G H Chambliss
Journal:  J Bacteriol       Date:  1974-12       Impact factor: 3.490
  8 in total

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