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Literature DB >> 4918240

Purification and chemistry of bacteriophage chi.

Abstract

From a stock of varkappa phage grown on Salmonella, a host-range mutant which attacks Escherichia coli was isolated. As in the case of Salmonella, only motile strains of E. coli are sensitive to varkappa. The phage shows an eclipse period of 35 min and a minimal latent period of 52 min. The adsorption rate constant is 3 x 10(-9) ml/min. Adsorption shows a marked dependence on temperature. Bacteriophage varkappa was purified by differential centrifugation and CsCl density gradient centrifugation. It contains deoxyribonucleic acid (DNA) which is double-stranded. The DNA has a molecular weight of 42 million and a guanine plus cytosine content of 57%. Of 68 molecules of DNA inspected, 7 were circular. The phage particle weight is about 90 million.

Entities: Chemical Species

Mesh：

Substances：
Culture Media
DNA, Viral

Year: 1967 PMID： 4918240 PMCID： PMC375286

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

22 in total

10. Bacteriophage DNA: correlation of buoyant density, melting temperature, and the chemically determined base composition.

Authors: A M Kropinski
Journal: J Virol Date: 1974-03 Impact factor: 5.103

Purification and chemistry of bacteriophage chi.

1. THE INITIAL STEPS IN INFECTION WITH COLIPHAGE M13.

2. DNA FROM BACTERIOPHAGE LAMBDA: MOLECULAR LENGTH AND CONFORMATION.

3. [Preparation and length measurements of the total desoxyribonucleic acid content of T2 bacteriophages].

4. A phage, phi chi, which attacks motile bacteria.

5. The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage lambda-dg.

6. [Early lysis produced by chloroform in bacteria infected by bacteriophage].

7. COHESION OF DNA MOLECULES ISOLATED FROM PHAGE LAMBDA.

8. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.

9. Assembly of phage lambda in vitro.

10. The mechanism of virus attachment to host cells. I. The role of ions in the primary reaction.

1. Flagellar determinants of bacterial sensitivity to chi-phage.

2. Ribonucleic acid polymerase mutant of Escherichia coli defective in flagella formation.

3. Identification of a bacterial sensing protein and effects of its elevated expression.

Review 4. Bacterial chemotaxis: the early years of molecular studies.

5. Two Escherichia coli chromosomal cistrons, sfrA and sfrB, which are needed for expression of F factor tra functions.

6. Isolation and characterization of motile Escherichia coli mutants resistant to bacteriophage chi.

7. [Adsorption of the bacteriophage Rp 1 to Rhodopseudomonas palustris 1 e 5].

Review 8. The structure of flagella and the genetic control of flagellation in Eubacteriales. A review.

9. Visualization of DNA in various phages (T4, chi, T7, phi 29) by ethidium bromide epi-fluorescent microscopy.

10. Bacteriophage DNA: correlation of buoyant density, melting temperature, and the chemically determined base composition.