Literature DB >> 4908530

Separation of Treponema pallidum from tissue debris through continuous-particle electrophoresis.

J D Schmale, D S Kellogg, C Miller, P Schammel, J D Thayer.   

Abstract

Treponema pallidum can only be cultured in living animal tissue, such as rabbit testes. However, the extract of these organisms from the testicular material leaves the T. pallidum contaminated with tissue debris. This paper describes the separation of T. pallidum from the debris by continuous-particle electrophoresis. The importance of equilibration time before electrophoresis is discussed.

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Mesh：

Year:  1970        PMID： 4908530      PMCID： PMC376669          DOI： 10.1128/am.19.2.287-289.1970

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  3 in total

1.  AN IMPROVED FTA TEST FOR SYPHILIS, THE ABSORPTION PROCEDURE (FTA-ABS).

Authors:  E F HUNTER; W E DEACON; P E MEYER
Journal:  Public Health Rep       Date:  1964-05       Impact factor: 2.792

2.  PURIFICATION OF THE PATHOGENIC TREPONEMA PALLIDUM BY DENSITY GRADIENT CENTRIFUGATION.

Authors:  T RATHLEV; C J PFAU
Journal:  Scand J Clin Lab Invest       Date:  1965       Impact factor: 1.713

3.  Treponema pallidum complement-fixation (TPCF) test for syphilis.

Authors:  J PORTNOY; H J MAGNUSON
Journal:  Am J Clin Pathol       Date:  1956-03       Impact factor: 2.493
  3 in total
  4 in total

1.  Percoll-purified Treponema pallidum, an improved fluorescent treponemal antibody-absorbed antigen.

Authors:  P A Hanff; C Fernandez; J D Folds
Journal:  J Clin Microbiol       Date:  1986-05       Impact factor: 5.948

2.  Isolation and purification of Treponema pallidum from syphilitic lesions in rabbits.

Authors:  P H Hardy; E E Nell
Journal:  Infect Immun       Date:  1975-06       Impact factor: 3.441

3.  Molecular characterization of common treponemal antigens.

Authors:  P A Hanff; J N Miller; M A Lovett
Journal:  Infect Immun       Date:  1983-05       Impact factor: 3.441

4.  The surface of virulent Treponema pallidum: resistance to antibody binding in the absence of complement and surface association of recombinant antigen 4D.

Authors:  J D Radolf; T E Fehniger; F J Silverblatt; J N Miller; M A Lovett
Journal:  Infect Immun       Date:  1986-05       Impact factor: 3.441
  4 in total

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