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Literature DB >> 4901472

Fluorometric quantitation of fluorescein-coupled antibodies attached to the cell membrane.

Abstract

A method was devised which allows the measurement, by direct and indirect fluorescence assay, of the binding of fluorescein-coupled antibodies directed against cell-membrane antigens. The systems used were: (1) species-specific antigens, (2) genetically determined isoantigens, (3) virus-determined, tumor-specific antigens, and (4) membrane-bound immunoglobulin in a Burkitt cell line. Titration of antibodies gave straight lines in the log versus log scale, the slope of the titration curve probably being dependent on the density of antigen receptors on the cell surface.

Entities: Chemical Disease

Mesh：

Substances：

Year: 1969 PMID： 4901472 PMCID： PMC223443 DOI： 10.1073/pnas.63.4.1157

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

7 in total

1. A REVISED NOMENCLATURE FOR THE HISTOCOMPATIBILITY-2 LOCUS OF THE MOUSE.

Authors: G D SNELL; G HOECKER; D B AMOS; J H STIMPFLING
Journal: Transplantation Date: 1964-11 Impact factor: 4.939

2. ANTIGENIC PROPERTIES OF LYMPHOMAS INDUCED BY THE MOLONEY AGENT.

Authors: E KLEIN; G KLEIN
Journal: J Natl Cancer Inst Date: 1964-03 Impact factor: 13.506

3. Fluorometric estimation of antigens (antibodies).

Authors: A R Neurath
Journal: Z Naturforsch B Date: 1965-10 Impact factor: 1.047

4. Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines.

Authors: E Klein; G Klein; J S Nadkarni; J J Nadkarni; H Wigzell; P Clifford
Journal: Cancer Res Date: 1968-07 Impact factor: 12.701

5. Direct membrane immunofluorescence reaction of Burkitt's lymphoma cells in culture.

Authors: G Goldstein; G Klein; G Pearson; P Clifford
Journal: Cancer Res Date: 1969-04 Impact factor: 12.701

6. Studies on Murine Sarcoma Virus: antigenic characterization of Murine Sarcoma Virus induced tumor cells.

Authors: J C Chuat; L Berman; P Gunvén; E Klein
Journal: Int J Cancer Date: 1969-07-15 Impact factor: 7.396

7. Chemical and physical variables affecting the properties of fluorescein isothiocyanate and its protein conjugates.

Authors: M R Klugerman
Journal: J Immunol Date: 1965-12 Impact factor: 5.422

7 in total

4 in total

Fluorometric quantitation of fluorescein-coupled antibodies attached to the cell membrane.

1. A REVISED NOMENCLATURE FOR THE HISTOCOMPATIBILITY-2 LOCUS OF THE MOUSE.

2. ANTIGENIC PROPERTIES OF LYMPHOMAS INDUCED BY THE MOLONEY AGENT.

3. Fluorometric estimation of antigens (antibodies).

4. Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines.

5. Direct membrane immunofluorescence reaction of Burkitt's lymphoma cells in culture.

6. Studies on Murine Sarcoma Virus: antigenic characterization of Murine Sarcoma Virus induced tumor cells.

7. Chemical and physical variables affecting the properties of fluorescein isothiocyanate and its protein conjugates.

1. A quantitative approach for immunofluorescence in microscopy: the use of antibody multilayers on nuclei.

2. Cell surface localized IgM-kappa immunoglobulin reactivity in a case of chronic lymphocytic leukaemia.

3. Fluorometric studies on the binding of antilymphocyte serum to lymph node cells.

4. Quantification of immunofluorescence on individual erythrocytes coated with varying amounts of antigen.