| Literature DB >> 490046 |
A Novak, J A Lowden, Y L Gravel, L S Wolfe.
Abstract
GM2 and GA2 gangliosides from the brain of a patient who died of Sandhoff's disease were purified by solvent partition, silicic acid and silica gel column chromatography, and silica gel preparative thin-layer chromatography. They were tritiated in the terminal N-acetylgalactosamine residue using galactose oxidase and sodium [3H]borohydride with the inclusion of catalase and peroxidase into the oxidation reaction. The specific activities were 4.62 X 10(8) dpm/mumol of GM2 ganglioside and 5.54 X 40(7) dpm/mumol of GA2 ganglioside. The addition of catalase and peroxidase to the tritiation procedure is recommended.Entities:
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Year: 1979 PMID： 490046
Source DB: PubMed Journal: J Lipid Res ISSN： 0022-2275 Impact factor: 5.922