| Literature DB >> 489989 |
Abstract
An enzyme-liked immunosorbent assay (ELISA) is described for determination of antibodies to collagen type I, II, III in serum or other body fluids. Polystyrene microplates are coated with either collagen I, II or III, and samples of body fluids being tested are incubated in the plates. Antibodies which attach specifically to the collagen are detected with anti-IgG antibody conjugated with peroxidase. Bound peroxidase is quantitatively estimated by the colour reaction produced with the substrate 5-aminosalicylic acid.Entities:
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Year: 1979 PMID: 489989 DOI: 10.1016/0022-1759(79)90127-3
Source DB: PubMed Journal: J Immunol Methods ISSN: 0022-1759 Impact factor: 2.303