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Literature DB >> 4897910

Isolation of adenyl cyclase from Escherichia coli.

Abstract

We have found a soluble cyclase, using for assay radioactively marked ATP as precursor. The reaction product was isolated by thin-layer chromatography and identified by specific degradation. After homogenization, part of the activity remained in the particulate fraction but could be easily extracted. The cyclase was concentrated 100-fold by conventional methods. The enzyme has a Mg(++) requirement and is inhibited by fluoride and inorganic pyrophosphate.

Entities: Chemical Gene Species

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Year: 1969 PMID： 4897910 PMCID： PMC534038 DOI： 10.1073/pnas.63.1.86

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

11 in total

1. Cyclic AMP as an antagonist of catabolite repression in Escherichia coli.

Authors: A Ullmann; J Monod
Journal: FEBS Lett Date: 1968-11 Impact factor: 4.124

2. ADENOSINE 3',5'-PHOSPHATE IN ESCHERICHIA COLI.

Authors: R S MAKMAN; E W SUTHERLAND
Journal: J Biol Chem Date: 1965-03 Impact factor: 5.157

3. Enzymatic hydrolysis of adenosine 3',5'-phosphoric acid.

Authors: G I DRUMMOND; S PERROTT-YEE
Journal: J Biol Chem Date: 1961-04 Impact factor: 5.157

4. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

5. Regulation of beta-galactosidase synthesis in Escherichia coli by cyclic adenosine 3',5'-monophosphate.

Authors: R L Perlman; I Pastan
Journal: J Biol Chem Date: 1968-10-25 Impact factor: 5.157

6. The role of the lac promotor locus in the regulation of beta-galactosidase synthesis by cyclic 3',5'-adenosine monophosphate.

Authors: I Pastan; R L Perlman
Journal: Proc Natl Acad Sci U S A Date: 1968-12 Impact factor: 11.205

7. Adenyl cyclase of Brevibacterium liquefaciens.

Authors: M Hirata; O Hayaishi
Journal: Biochim Biophys Acta Date: 1967-11-21

8. Cyclic 3'5-AMP: stimulation of beta-galactosidase and tryptophanase induction in E. coli.

Authors: R Perlman; I Pastan
Journal: Biochem Biophys Res Commun Date: 1968-03-27 Impact factor: 3.575

9. Adenosine 3',5'-phosphate in biological materials. I. Purification and properties of cyclic 3',5'-nucleotide phosphodiesterase and use of this enzyme to characterize adenosine 3',5'-phosphate in human urine.

Authors: R W BUTCHER; E W SUTHERLAND
Journal: J Biol Chem Date: 1962-04 Impact factor: 5.157

10. OCCURRENCE OF NUCLEOTIDES IN CULTURE FLUIDS OF MICROORGANISMS. V. EXCRETION OF ADENOSINE CYCLIC 3',5'-PHOSPHATE BY BREVIBACTERIUM LIQUEFACIENS SP. N.

Authors: T OKABAYASHI; A YOSHIMOTO; M IDE
Journal: J Bacteriol Date: 1963-11 Impact factor: 3.490

28 in total

1. Combinatorial transcriptional control of the lactose operon of Escherichia coli.

Authors: Thomas Kuhlman; Zhongge Zhang; Milton H Saier; Terence Hwa
Journal: Proc Natl Acad Sci U S A Date: 2007-03-21 Impact factor: 11.205

2. Cyclic 3', 5'-adenosine monophosphate phosphodiesterase mutants of Salmonella typhimurium.

Authors: M D Alper; B N Ames
Journal: J Bacteriol Date: 1975-06 Impact factor: 3.490

3. Regulation of cyclic AMP levels in Arthrobacter crystallopoietes and a morphogenetic mutant.

Authors: R W Hamilton; P E Kolenbrander
Journal: J Bacteriol Date: 1978-06 Impact factor: 3.490

Review 4. Cyclic adenosine 5'-monophosphate in Escherichia coli.

Authors: I Pastan; S Adhya
Journal: Bacteriol Rev Date: 1976-09

5. A convenient colourimetric method for routine assay of brain adenylate cyclase.

Authors: I A Macdonald
Journal: Experientia Date: 1974-12-15

6. [Cyclic nucleotides as intracellular transmitter substances in hormone actions].

Authors: G Schultz
Journal: Klin Wochenschr Date: 1971-10-01

7. Binding of adenosine 3':5'-cyclic phosphate to G factor of Escherichia coli, and its effects on GTPase, RNase V, and protein synthesis.

Authors: M Kuwano; D Schlessinger
Journal: Proc Natl Acad Sci U S A Date: 1970-05 Impact factor: 11.205