| Literature DB >> 4889266 |
Abstract
A simple and rapid method is described for separation of T-even bacteriophage deoxyribonucleic acid (DNA) from host (Escherichia coli) DNA by hydroxyapatite column chromatography with a shallow gradient of phosphate buffer at neutral pH. By this method, bacteriophage T2, T4, and T6 DNA (but not T5, T7, or lambda DNA) could be separated from host E. coli DNA. It was found that glucosylation of the T-even phage DNA is an important factor in separation.Entities:
Mesh：
Substances：
Year: 1969 PMID： 4889266 PMCID： PMC249910 DOI： 10.1128/jb.98.1.104-108.1969
Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490