Literature DB >> 4886674

Immunological detection of single amino acid substitutions in alkaline phosphatase.

G T Cocks, A C Wilson.   

Abstract

Antiserums to wild-type alkaline phosphatase from Escherichia coli were prepared and tested for reactivity with phosphatases altered by point mutations. Eight out of the nine mutant enzymes were distinguished from the wild type with quantitative microcomplement fixation. The structural changes are among the smallest yet observed immunologically.

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Year:  1969        PMID： 4886674     DOI： 10.1126/science.164.3876.188

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  14 in total

1.  Antigenic comparison of swine influenza virus isolates.

Authors:  D M Nath; L S Rodkey; H C Minocha
Journal:  Arch Virol       Date:  1975       Impact factor: 2.574

2.  Mutation affecting plasmolysis in Escherichia coli.

Authors:  H J Wijsman
Journal:  J Bacteriol       Date:  1972-05       Impact factor: 3.490

3.  Enzyme evolution in the Enterobacteriaceae.

Authors:  G T Cocks; A C Wilson
Journal:  J Bacteriol       Date:  1972-06       Impact factor: 3.490

4.  Serological relatedness of bacterial deoxyribonucleic acid polymerases.

Authors:  S W Tafler; P Setlow; L Levine
Journal:  J Bacteriol       Date:  1973-01       Impact factor: 3.490

5.  [Quantitative structure comparison of protein surfaces by micro-complement fixation].

Authors:  H Haas; R Zwilling
Journal:  Naturwissenschaften       Date:  1976-03

6.  Aldolase of lactic acid bacteria: a case history in the use of an enzyme as an evolutionary marker.

Authors:  J London; K Kline
Journal:  Bacteriol Rev       Date:  1973-12

7.  Immunological comparison of azurins of known amino acid sequence. Dependence of cross-reactivity upon sequence resemblance.

Authors:  A B Champion; K L Soderberg; A C Wilson
Journal:  J Mol Evol       Date:  1975-09-08       Impact factor: 2.395

8.  Preparation of specific antisera to Drosophila acid phosphatase without rigorous protein purification.

Authors:  N Arnheim; R MacIntyre
Journal:  Biochem Genet       Date:  1976-04       Impact factor: 1.890

9.  Murine type-C virus group-specific antigens: interstrain immunochemical, biophysical, and amino acid sequence differences.

Authors:  S Oroszlan; M R Summers; C Foreman; R V Gilden
Journal:  J Virol       Date:  1974-12       Impact factor: 5.103

10.  Microcomplement fixation studies on the evolution of alpha-glycerophosphate dehydrogenase within the genus Drosophila.

Authors:  G E Collier; R J MacIntyre
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205
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