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Literature DB >> 4877123

Extraction and isolation of individual ribosomal proteins from Escherichia coli.

Abstract

We have described a new method for the quantitative separation of ribosomal proteins and ribosomal ribonucleic acid. A procedure for the preparation of individual ribosomal proteins by polyacrylamide gel electrophoresis is also described. By the use of gels with smaller pores, at least four of the electrophoretic components from the 30S ribosome can be split into additional protein fractions. By the methods described here, it is possible to isolate in high purity at least 15 different proteins from the 30S ribosome of Escherichia coli.

Entities: Chemical Species

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Year: 1968 PMID： 4877123 PMCID： PMC252306 DOI： 10.1128/jb.96.2.358-364.1968

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

15 in total

4. Ribosomal vaccines. II. Specificity of the immune response to ribosomal ribonucleic acid and protein isolated from Salmonella typhimurium.

Authors: W Johnson
Journal: Infect Immun Date: 1973-09 Impact factor: 3.441

5. Gonococcal ribosomes as skin test antigens. II. Precision Of the Method, Attempts To Identify The Ribosomal Antigen, and Correlation with The Macrophage Migration Inhibition test.

Authors: R C Judd; W L Koostra
Journal: Br J Vener Dis Date: 1976-02

10. Kinetics of Haemophilus influenzae type B infection in normal and ribosome-immunized mice using intraperitoneal and intracerebral routes of inoculation.

Authors: J P Burans; F H Kruszewski; M Lynn; M Solotorovsky
Journal: Br J Exp Pathol Date: 1981-10

Extraction and isolation of individual ribosomal proteins from Escherichia coli.

1. FRACTIONATION OF THE RIBOSOMAL PROTEIN FROM ESCHERICHIA COLI.

2. Studies on the composition of the protein from Escherichia coli ribosomes.

3. CHLORAMPHENICOL-PROMOTED REPRESSION OF beta-GALACTOSIDASE SYNTHESIS IN ESCHERICHIA COLI.

4. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.

5. Amino-acid sequence of horse heart cytochrome c.

6. Chemical basis for heterogeneity of ribosomal proteins.

7. Fractionation and some properties of the ribosomal proteins in Escherichia coli ribosomes.

8. Ribosomal proteins of Escherichia coli. I. Demonstration of different primary structures.

9. Isolation and characterization of ribonuclease I mutants of Escherichia coli.

10. The requirements for specific sRNA binding by ribosomes.

1. Bacterial ribosome.

2. Structural heterogeneity of 30S and 50S ribosomal subunits in Bacillus licheniformis.

3. The Chloroplast and Cytoplasmic Ribosomes of Euglena: II. Characterization of Ribosomal Proteins.

4. Ribosomal vaccines. II. Specificity of the immune response to ribosomal ribonucleic acid and protein isolated from Salmonella typhimurium.

5. Gonococcal ribosomes as skin test antigens. II. Precision Of the Method, Attempts To Identify The Ribosomal Antigen, and Correlation with The Macrophage Migration Inhibition test.

6. Immunogenicity of ribosomal vaccines isolated from group A, type 14 Streptococcus pyogenes.

7. Isolation and amino acid composition of ribosomal proteins from Bacillus stearothermophilus.

8. Chromosomal location of ribosomal protein cistrons determined by intergeneric bacterial mating.

9. Ribosomes, polyribosomes, and deoxyribonucleic acid from thermophilic mesophilic, and psychrophilic clostridia.

10. Kinetics of Haemophilus influenzae type B infection in normal and ribosome-immunized mice using intraperitoneal and intracerebral routes of inoculation.