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Literature DB >> 4876099

The fractionation of nuclei from mammalian cells by zonal centrifugation.

I R Johnston, A P Mathias, F Pennington, D Ridge.

Abstract

1. Purified liver nuclei from adult rats separate into two main zones when centrifuged in the slow-speed zonal rotor. One zone contains diploid nuclei, the other tetraploid. 2. The effect of age on the pattern of rat liver ploidy was examined. Tetraploid nuclei are virtually absent from young animals. They increase in proportion steadily with age. Partial hepatectomy disturbs the pattern of ploidy. 3. The zonal centrifuge permits the separation of diploid, tetraploid, octaploid and hexadecaploid nuclei from mouse liver. 4. Rat liver nuclei are isopycnic with sucrose solutions of density 1.35 at 5 degrees .

Entities: Chemical Species

Mesh：

Year: 1968 PMID： 4876099 PMCID： PMC1186761 DOI： 10.1042/bj1090127

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

11 in total

1. Fractionation of rat liver nuclei into diploid and tetraploid DNA classes by continuous density gradient sedimentation.

Authors: J A FALZONE; C H BARROWS; M J YIENGST
Journal: Exp Cell Res Date: 1962-03 Impact factor: 3.905

1. Enzymes involved in adenosine metabolism in rat white and brown adipocytes. Effects of streptozotocin-diabetes, hypothyroidism, age and sex differences.

Authors: Z Jamal; E D Saggerson
Journal: Biochem J Date: 1987-08-01 Impact factor: 3.857

2. The distribution of some hydrolases in glomeruli and tubular fragments prepared from rat kidney by zonal centrifugation.

Authors: D G Taylor; R G Price; D Robinson
Journal: Biochem J Date: 1971-05 Impact factor: 3.857

6. Evidence for the rapid direct control both in vivo and in vitro of the efficiency of oxidative phosphorylation by 3,5,3'-tri-iodo-L-thyronine in rats.

Authors: R Palacios-Romero; J Mowbray
Journal: Biochem J Date: 1979-12-15 Impact factor: 3.857

7. Fractionation of nuclei from brain by zonal centrifugation and a study of the ribonucleic acid polymerase activity in the various classes of nuclei.

Authors: J Austoker; D Cox; A P Mathias
Journal: Biochem J Date: 1972-10 Impact factor: 3.857

8. Ligation and synthesis of chromatin deoxyribonucleic acid in vitro in neuronal, glial and liver nuclei isolated from adult guinea pig.

Authors: N Inoue; T Ono; T Kato
Journal: Biochem J Date: 1979-06-15 Impact factor: 3.857

9. The variation with age of the structure of chromatin in three cell types from rat liver.

Authors: V Zongza; A P Mathias
Journal: Biochem J Date: 1979-05-01 Impact factor: 3.857

10. Proteins from different classes of liver nuclei in normal and thioacetamide-treated rats.

Authors: F Gonzalez-Mujica; A P Mathias
Journal: Biochem J Date: 1973-07 Impact factor: 3.857

The fractionation of nuclei from mammalian cells by zonal centrifugation.

1. Fractionation of rat liver nuclei into diploid and tetraploid DNA classes by continuous density gradient sedimentation.

2. The growth of cell populations and the properties in tissue culture of regenerating liver of the rat.

3. RNA synthesis in intact rat liver nuclei.

4. A procedure for the isolation of enzymically active rat-liver nuclei.

Review 5. Zonal centrifuges and other separation systems.

6. Fractionation of rat liver nuclei by sucrose gradient centrifugation.

7. The nuclear pattern of the parenchymal cells of the liver after partial hepatectomy.

8. Studies on the stimulation by ammonium sulphate of the DNA-dependent RNA polymerase of isolated rat-liver nuclei.

9. Nuclear ploidy in mammalian parenchymal liver cells.

10. Density gradient isolation of rat liver nuclei with high DNA content.