| Literature DB >> 487209 |
J J Warsh, A Chiu, D D Godse, D V Coscina.
Abstract
A simplified assay is described for measurement of picogram amounts of serotonin (5-HT) by high pressure liquid chromatography with electrochemical detection (LCEC). The procedure involves pre-purification of brain 5-HT by adsorption on Amberlite CG-50. Serotonin is subsequently resolved and detected by LCEC on Zipax SCX resin. The present method gives working sensitivities of at least 100 pg, tissue recoveries of 95% and very low interassay variability (coefficient of variation = 3%). Determination of rat brain area 5-HT by this LCEC method is described and compared to other high sensitivity methods.Entities:
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Year: 1979 PMID： 487209 DOI： 10.1016/0361-9230(79)90043-1
Source DB: PubMed Journal: Brain Res Bull ISSN： 0361-9230 Impact factor: 4.077