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Literature DB >> 4868861

Rapid method for characterization of actinomycetes by cell wall composition.

Abstract

To achieve a rapid identification of the cell wall components of actinomycetes, several modifications of the procedure of Cummins and Harris were developed. Purified cell walls were prepared by extraction of whole cells with 0.1 N NaOH. A 2-hr 2 N HCl hydrolysate was prepared for identification of sugars. A 2-hr 6 N HCl hydrolysate was prepared for amino acid analysis. Two-dimensional paper chromatography was run on 19-cm papers. Total time required for a single analysis was approximately 24 hr. This procedure gave qualitative results which were completely satisfactory for differentiation of certain species in the genus Actinomyces and in related genera.

Entities: Chemical

Mesh：

Substances：

Year: 1968 PMID： 4868861 PMCID： PMC547396 DOI： 10.1128/am.16.2.279-284.1968

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

13 in total

4. The stereoisomers of alpha epsilon-diaminopimelic acid. II. Their distribution in the bacterial order Actinomycetales and in certain Eubacteriales.

Authors: D S HOARE; E WORK
Journal: Biochem J Date: 1957-03 Impact factor: 3.857

5. Studies on the cell-wall composition and taxonomy of Actinomycetales and related groups.

Authors: C S CUMMINS; H HARRIS
Journal: J Gen Microbiol Date: 1958-02

6. The chemical composition of the cell wall in some gram-positive bacteria and its possible value as a taxonomic character.

Authors: C S CUMMINS; H HARRIS
Journal: J Gen Microbiol Date: 1956-07

7. Chemical and antigenic studies on cell walls of mycobacteria, corynebacteria, and nocardias.

Authors: C S Cummins
Journal: Am Rev Respir Dis Date: 1965-12

8. Identification of Actinomyces israelii and Actinomyces naeslundii by fluorescent-antibody and agar-gel diffusion techniques.

Authors: F W Lambert; J M Brown; L K Georg
Journal: J Bacteriol Date: 1967-11 Impact factor: 3.490

9. Comparative cell wall analyses of morphological forms within the genus Actinomyces.

Authors: L Pine; C J Boone
Journal: J Bacteriol Date: 1967-10 Impact factor: 3.490

10. Studies on the chemical structure of the streptococcal cell wall. I. The identification of a mucopeptide in the cell walls of groups A and A-variant streptococci.

Authors: R M KRAUSE; M MCCARTY
Journal: J Exp Med Date: 1961-07-01 Impact factor: 14.307

14 in total

1. Immunological demonstration of a unique 3,4-dihydroxyphenylacetate 2,3-dioxygenase in soil Arthrobacter strains.

Authors: P E Olson; B Qi; L Que; L P Wackett
Journal: Appl Environ Microbiol Date: 1992-09 Impact factor: 4.792

2. Cellulolytic cocci isolated from the cecum of guinea pigs (Cavia porcellus).

Authors: B A Dehority
Journal: Appl Environ Microbiol Date: 1977-06 Impact factor: 4.792

3. Predominant Catalase-negative Soil Bacteria. III. Agromyces, gen. n., Microorganisms Intermediary to Actinomyces and Nocardia.

Authors: W E Gledhill; L E Casida
Journal: Appl Microbiol Date: 1969-09

10. Identification of Actinomyces, Arachnia, Bacterionema, Rothia, and Propionibacterium species by defined immunofluorescence.

Authors: K Holmberg; U Forsum
Journal: Appl Microbiol Date: 1973-05

Rapid method for characterization of actinomycetes by cell wall composition.

1. IDENTIFICATION OF SPECIES OF ACTINOMYCES.

2. COMPARISON OF THE CELL-WALL COMPOSITION OF MORPHOLOGICALLY DISTINCT ACTINOMYCETES.

3. Characterization of a propionic acid producing actinomycete, Actinomyces propionicus, sp. nov.

4. The stereoisomers of alpha epsilon-diaminopimelic acid. II. Their distribution in the bacterial order Actinomycetales and in certain Eubacteriales.

5. Studies on the cell-wall composition and taxonomy of Actinomycetales and related groups.

6. The chemical composition of the cell wall in some gram-positive bacteria and its possible value as a taxonomic character.

7. Chemical and antigenic studies on cell walls of mycobacteria, corynebacteria, and nocardias.

8. Identification of Actinomyces israelii and Actinomyces naeslundii by fluorescent-antibody and agar-gel diffusion techniques.

9. Comparative cell wall analyses of morphological forms within the genus Actinomyces.

10. Studies on the chemical structure of the streptococcal cell wall. I. The identification of a mucopeptide in the cell walls of groups A and A-variant streptococci.

1. Immunological demonstration of a unique 3,4-dihydroxyphenylacetate 2,3-dioxygenase in soil Arthrobacter strains.

2. Cellulolytic cocci isolated from the cecum of guinea pigs (Cavia porcellus).

3. Predominant Catalase-negative Soil Bacteria. III. Agromyces, gen. n., Microorganisms Intermediary to Actinomyces and Nocardia.

Review 4. Peptidoglycan types of bacterial cell walls and their taxonomic implications.

5. Amino acid composition of treponemes.

Review 6. Taxonomy and clinical significance of Actinomycetaceae and Propionibacteriaceae.

7. Degradation of organic nitrogenous wastes by a soil streptomycete.

8. Isolation and characterization of a pentachlorophenol-degrading bacterium.

9. Quantitative analysis of Actinomyces cell walls.

10. Identification of Actinomyces, Arachnia, Bacterionema, Rothia, and Propionibacterium species by defined immunofluorescence.