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Literature DB >> 4868351

Screening of Escherichia coli temperature-sensitive mutants by pretreatment with glucose starvation.

D Boyd, R Nixon, S Gillespie, D Gillespie.

Abstract

A system for screening Escherichia coli temperature-sensitive mutants is described. The system involves glucose starvation and minimizes ambiguities introduced by the interdependencies of macromolecular synthesis during balanced growth. The system permits the quick recognition of protein synthesis mutants and their classification into two general catagories. Complete protein synthesis mutants are unable to make any polypeptide material, whereas partial protein synthesis mutants are able to produce inactive proteins. The phenotypes of several mutants are described.

Entities: Disease Species

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Year: 1968 PMID： 4868351 PMCID： PMC252129 DOI： 10.1128/jb.95.3.1040-1050.1968

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

25 in total

1. MAPPING OF TEMPERATURE-SENSITIVE MUTANTS IN BACTERIOPHAGE T5.

Authors: W D FATTIG; F LANNI
Journal: Genetics Date: 1965-01 Impact factor: 4.562

2. [On a thermosensitive repression system in the Escherichia coli lambda bacteriophage].

Authors: R SUSSMAN; F JACOB
Journal: C R Hebd Seances Acad Sci Date: 1962-02-19

3. Studies on a mutant strain of Escherichia coli which requires both methionine and lysine for growth.

Authors: K J BACK; E G WESTAWAY
Journal: J Gen Microbiol Date: 1962-01

4. Genetic regulatory mechanisms in the synthesis of proteins.

Authors: F JACOB; J MONOD
Journal: J Mol Biol Date: 1961-06 Impact factor: 5.469

5. Genetic Control of Biochemical Reactions in Neurospora.

Authors: G W Beadle; E L Tatum
Journal: Proc Natl Acad Sci U S A Date: 1941-11-15 Impact factor: 11.205

6. A genetic study of temperature-sensitive mutants of the subtilis phage SP82.

Authors: E Kahan
Journal: Virology Date: 1966-12 Impact factor: 3.616

7. [Thermosensitive mutants of Escherichia coli K 12. I. Isolation and rapid characterization].

Authors: M Kohiyama; D Cousin; A Ryter; F Jacob
Journal: Ann Inst Pasteur (Paris) Date: 1966-04

8. Temperature-sensitive mutation in Paramecium aurelia. I. Induction and inheritance.

Authors: S Igarashi
Journal: Mutat Res Date: 1966-02 Impact factor: 2.433

9. PROTEIN AND NUCLEIC ACID SYNTHESIS IN TWO MUTANTS OF ESCHERICHIA COLI WITH TEMPERATURE-SENSITIVE AMINOACYL RIBONUCLEIC ACID SYNTHETASES.

Authors: L EIDLIC; F C NEIDHARDT
Journal: J Bacteriol Date: 1965-03 Impact factor: 3.490

Screening of Escherichia coli temperature-sensitive mutants by pretreatment with glucose starvation.

1. MAPPING OF TEMPERATURE-SENSITIVE MUTANTS IN BACTERIOPHAGE T5.

2. [On a thermosensitive repression system in the Escherichia coli lambda bacteriophage].

3. Studies on a mutant strain of Escherichia coli which requires both methionine and lysine for growth.

4. Genetic regulatory mechanisms in the synthesis of proteins.

5. Genetic Control of Biochemical Reactions in Neurospora.

6. A genetic study of temperature-sensitive mutants of the subtilis phage SP82.

7. [Thermosensitive mutants of Escherichia coli K 12. I. Isolation and rapid characterization].

8. Temperature-sensitive mutation in Paramecium aurelia. I. Induction and inheritance.

9. PROTEIN AND NUCLEIC ACID SYNTHESIS IN TWO MUTANTS OF ESCHERICHIA COLI WITH TEMPERATURE-SENSITIVE AMINOACYL RIBONUCLEIC ACID SYNTHETASES.

10. Macromolecule synthesis in temperature-sensitive mutants of yeast.

1. Employing site-specific recombination for conditional genetic analysis in Sinorhizobium meliloti.

2. Effect of elevated temperatures on protein synthesis in Escherichia coli.

3. Deductive analysis of a protein-synthesis mutant of Escherichia coli.

4. Metabolic events occurring during recovery from prolonged glucose starvation in Escherichia coli.

5. A new RNA synthesis mutant of E. coli.

6. Studies of sulfate utilization by algae: 8. The ubiquity of sulfate reduction to thiosulfate.

7. New rifampin-resistant mutant of Escherichia coli.