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Literature DB >> 4864403

Identification of Mycoplasma and other microorganisms by polyacrylamide-gel electrophoresis of cell proteins.

Abstract

The proteins of Mycoplasma cells of various species produce highly reproducible and species-specific electrophoretic patterns in polyacrylamide gels containing 5 m urea and 35% acetic acid. These electrophoretic patterns can be used for the rapid identification and classification of Mycoplasma. Preliminary results indicate that this method may also be used for the identification and classification of other microorganisms.

Entities: Chemical Disease

Mesh：

Substances：

Year: 1967 PMID： 4864403 PMCID： PMC276908 DOI： 10.1128/jb.94.6.1807-1810.1967

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

16 in total

1. Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO.

Authors: R M CHANOCK; L HAYFLICK; M F BARILE
Journal: Proc Natl Acad Sci U S A Date: 1962-01-15 Impact factor: 11.205

2. The isolation of organisms of the pleuropneumonia group from dogs.

Authors: D G EDWARD; W A FITZGERALD
Journal: J Gen Microbiol Date: 1951-08

3. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

4. Studies on the electron transfer system. LXVII. Polyacrylamide gel electrophoresis of the mitochondrial electron transfer complexes.

Authors: K Takayama; D H MacLennan; A Tzagoloff; C D Stoner
Journal: Arch Biochem Biophys Date: 1966-04 Impact factor: 4.013

Review 10. Structure of biological membranes.

Authors: E D Korn
Journal: Science Date: 1966-09-23 Impact factor: 47.728

27 in total

1. Comparative electrophoretic and amino acid analyses of isolated membranes from Streptococcus pyogenes and stabilized L-form.

Authors: C Panos; G Fagan; C G Zarkadas
Journal: J Bacteriol Date: 1972-10 Impact factor: 3.490

Review 2. Cell biology of the mycoplasmas.

Authors: J Maniloff; H J Morowitz
Journal: Bacteriol Rev Date: 1972-09

3. Acrylamide gel electrophoresis of proteins of Neisseria gonorrhoeae as an epidemiological tool.

Authors: R C Noble; S C Schell
Journal: Infect Immun Date: 1978-01 Impact factor: 3.441

4. Light-induced Changes in the Pattern of Protein Synthesis during the Early Stages of Greening of Etiolated Maize Leaves.

Authors: D Kaveh; E Harel
Journal: Plant Physiol Date: 1973-04 Impact factor: 8.340

Identification of Mycoplasma and other microorganisms by polyacrylamide-gel electrophoresis of cell proteins.

1. Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO.

2. The isolation of organisms of the pleuropneumonia group from dogs.

3. Protein measurement with the Folin phenol reagent.

4. Studies on the electron transfer system. LXVII. Polyacrylamide gel electrophoresis of the mitochondrial electron transfer complexes.

5. Genetic differentiation by nucleic acid homology. 3. Relationships among mycoplasma, L-forms, and bacteria.

6. Recommendations on nomenclature of the order Mycoplasmatales.

7. Electrophoretic patterns of membrane proteins of Mycoplasma.

8. DNA homology and taxonomy of Pseudomonas and Xanthomonas.

9. Compraison of mycoplasmas associated with human tumors, leukemia, and tissue cultures.

Review 10. Structure of biological membranes.

1. Comparative electrophoretic and amino acid analyses of isolated membranes from Streptococcus pyogenes and stabilized L-form.

Review 2. Cell biology of the mycoplasmas.

3. Acrylamide gel electrophoresis of proteins of Neisseria gonorrhoeae as an epidemiological tool.

4. Light-induced Changes in the Pattern of Protein Synthesis during the Early Stages of Greening of Etiolated Maize Leaves.

5. Isolation and characterization of Mycoplasma conjunctivae sp. n. from sheep and goats with keratoconjunctivitis.

6. Zone electrophoresis of enzymes in bacterial taxonomy.

7. Mycoplasma taxonomy studiedy electrophoresis of cell proteins.

8. Physiological and serological comparisons among strains of Mycoplasma granularum and Mycoplasma laidlawii.

9. Purification and serological characterization of a type-specific antigen of Streptococcus equisimilis.

10. Isoelectric focusing of mycoplasma proteins.