Literature DB >> 486092

Immobilized-enzyme pipette. Scope and limitations of a simple device.

P V Sundaram.   

Abstract

Disposable pipette tips made of polymeric nylon tube with enzymes bound covalently to their inside surface and fixed to the stem of an automatic, adjustable-volume pipette holder together constitutes an immobilized enzyme pipette or 'Impette'. The present paper describes the application in research laboratories and clinics of this new development, with urease as an example in the determination of blood urea.

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Year:  1979        PMID： 486092      PMCID： PMC1186643          DOI： 10.1042/bj1790445

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  6 in total

1.  Preparation and properties of urease chemically attached to nylon tube.

Authors:  P V. Sundaram; W E. Hornby
Journal:  FEBS Lett       Date:  1970-10       Impact factor: 4.124

2.  Modified reagents for determination of urea and ammonia.

Authors:  A L CHANEY; E P MARBACH
Journal:  Clin Chem       Date:  1962-04       Impact factor: 8.327

3.  An activated enzyme electrode for creatinine.

Authors:  M Meyerhoff; G A Rechnitz
Journal:  Anal Chim Acta       Date:  1976-09       Impact factor: 6.558

4.  An enzyme electrode for the amperometric determination of glucose.

Authors:  G G Guilbault; G J Lubrano
Journal:  Anal Chim Acta       Date:  1973-05       Impact factor: 6.558

5.  Immobilized-enzyme nylon-tube reactor for routine determination of uric acid in serum.

Authors:  P V Sundaram; M P Igloi; R Wassermann; W Hinsch
Journal:  Clin Chem       Date:  1978-10       Impact factor: 8.327

6.  Immobilized-enzyme nylon-tube reactors for routine determination of urea and citrulline in serum.

Authors:  P V Sundaram; M P Igloi; R Wassermann; W Hinsch; K J Knoke
Journal:  Clin Chem       Date:  1978-02       Impact factor: 8.327
  6 in total

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