Literature DB >> 4855151

The use of radiolabelled T1 ribonuclease to monitor the efficiency of Edman degradations.

D J McKean, G P Smith.   

Abstract

A simple radiolabel tracer method is presented for monitoring the efficiency of the first ten cycles of an Edman degradation. This method consists of adding a very small amount of radiolabelled S-carboxymethylated T(1) ribonuclease to the protein to be sequenced, and counting the radioactivity of a small sample from each of the degradative cycles.

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Year:  1974        PMID： 4855151      PMCID： PMC1166342          DOI： 10.1042/bj1390779

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  2 in total

1.  Quantitative procedures for use with the Edman-Begg sequenator. Partial sequences of two unusual immunoglobulin light chains, Rzf and Sac.

Authors:  O Smithies; D Gibson; E M Fanning; R M Goodfliesh; J G Gilman; D L Ballantyne
Journal:  Biochemistry       Date:  1971-12-21       Impact factor: 3.162

2.  The structure and function of ribonuclease T 1 . XVII. Isolation and amino acid sequences of papain and subtilisin peptides from ribonuclease T 1 --the complete covalent structure of ribonuclease T 1 .

Authors:  K Takahashi
Journal:  J Biochem       Date:  1971-12       Impact factor: 3.387
  2 in total
  2 in total

1.  Mechanisms of antibody diversity: multiple genes encode structurally related mouse kappa variable regions.

Authors:  D J McKean; M Bell; M Potter
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

2.  Isolation and partial characterization of human parotid basic proteins.

Authors:  E H Peters; E A Azen
Journal:  Biochem Genet       Date:  1977-10       Impact factor: 1.890
  2 in total

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