Characterisation and quantitation of morphine in urine using high-pressure liquid chromatography with fluorescence detection.

A simple and rapid method for the identification and quantitation of morphine in urine samples is described. The procedure, which involves conversion of the drug a fluorescent product followed by liquid chromatography, is shown to be highly sensitive and specific. Levels down to 0.01 mug/ml of morphine can be quantitatively detected in urine. A large number of drugs have been tested and shown not to interfere.