Literature DB >> 4832756

Determination of hepatic cholesterol 7alpha-hydroxylase activity in man.

F J Schmitz, F J McDonald.   

Abstract

Methods were developed to determine the activity of the microsomal enzyme cholesterol 7alpha-hydroxylase in human liver. The enzyme assay could be performed with as little as 20 mg of fresh liver tissue, thus making the procedure applicable to specimens obtained by percutaneous liver biopsy. Optimal assay conditions were determined and the identity and radioactive purity of the reaction product, cholest-5-ene-3beta,7alpha-diol (7alpha-hydroxycholesterol) were established. Specific enzyme activity was measured in a number of patients with disorders of lipid metabolism.

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Year:  1974        PMID： 4832756

Source DB:  PubMed          Journal:  J Lipid Res        ISSN： 0022-2275            Impact factor:   5.922


  10 in total

1.  Marked variability in hepatic expression of cytochromes CYP7A1 and CYP27A1 as compared to cerebral CYP46A1. Lessons from a dietary study with omega 3 fatty acids in hamsters.

Authors:  Natalia Mast; Marjan Shafaati; Wahiduz Zaman; Wenchao Zheng; Deborah Prusak; Thomas Wood; G A S Ansari; Anita Lövgren-Sandblom; Maria Olin; Ingemar Bjorkhem; Irina Pikuleva
Journal:  Biochim Biophys Acta       Date:  2010-03-16

2.  Normal antipyrine metabolism in patients with cholesterol cholelithiasis. Evidence that the disease is not due to generalized hepatic microsomal dysfunction.

Authors:  G W Hepner; E S Vesell
Journal:  Am J Dig Dis       Date:  1975-01

Review 3.  Bile acid biosynthesis. Pathways and regulation.

Authors:  E H Mosbach; G Salen
Journal:  Am J Dig Dis       Date:  1974-10

Review 4.  Cholesterol-metabolizing cytochromes P450: implications for cholesterol lowering.

Authors:  Irina A Pikuleva
Journal:  Expert Opin Drug Metab Toxicol       Date:  2008-11       Impact factor: 4.481

5.  A case of cerebrotendinous xanthomatosis. II: The sterol content of a cataractous lens.

Authors:  P McKenna; S J Morgan; R C Bosanquet; M F Laker
Journal:  Br J Ophthalmol       Date:  1990-10       Impact factor: 4.638

Review 6.  Ursodeoxycholic acid: a review of its pharmacological properties and therapeutic efficacy.

Authors:  A Ward; R N Brogden; R C Heel; T M Speight; G S Avery
Journal:  Drugs       Date:  1984-02       Impact factor: 9.546

7.  Hepatic cholesterol and bile acid synthesis in Japanese patients with cholesterol gallstones.

Authors:  A Honda; T Yoshida; N Tanaka; Y Matsuzaki; B He; T Osuga; N Kobayashi; K Ozawa
Journal:  Gastroenterol Jpn       Date:  1993-06

8.  Comparative effects of cholestanol and cholesterol on hepatic sterol and bile acid metabolism in the rat.

Authors:  S Shefer; S Hauser; G Salen; F G Zaki; J Bullock; E Salgado; J Shevitz
Journal:  J Clin Invest       Date:  1984-11       Impact factor: 14.808

9.  Competitive inhibition of bile acid synthesis by endogenous cholestanol and sitosterol in sitosterolemia with xanthomatosis. Effect on cholesterol 7 alpha-hydroxylase.

Authors:  S Shefer; G Salen; L Nguyen; A K Batta; V Packin; G S Tint; S Hauser
Journal:  J Clin Invest       Date:  1988-12       Impact factor: 14.808

10.  A novel pathway for biosynthesis of cholestanol with 7 alpha-hydroxylated C27-steroids as intermediates, and its importance for the accumulation of cholestanol in cerebrotendinous xanthomatosis.

Authors:  S Skrede; I Björkhem; M S Buchmann; G Hopen; O Fausa
Journal:  J Clin Invest       Date:  1985-02       Impact factor: 14.808
  10 in total

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